Research Data Leeds Repository

Lipid coated liquid crystal droplets for the on-chip detection of Antimicrobial peptides  - dataset

Bao, Peng (2019) Lipid coated liquid crystal droplets for the on-chip detection of Antimicrobial peptides  - dataset. University of Leeds. [Dataset] https://doi.org/10.5518/466

Dataset description

We describe a novel biosensor based on phospholipid-coated nematic liquid crystal (LC) droplets and demonstrate the detection of Smp43, a model antimicrobial peptide (AMP) from the venom of North African scorpion Scorpio maurus palmatus. Mono-disperse lipid-coated LC droplets of diameter 16.7 ± 0.2 μm were generated using PDMS microfluidic devices with a flow-focusing configuration and were the target for AMPs. The droplets were trapped in a bespoke microfluidic trap structure and were simultaneously treated with Smp43 at gradient concentrations in six different chambers. The disruption of the lipid monolayer by the Smp43 was detected (< 6 μM) at concentrations well within its biologically active range, indicated by a dramatic change in the appearance of the droplets associated with the transition from a typical radial configuration to a bipolar configuration, which is readily observed by polarizing microscopy. This suggests the system has feasibility as a drug-discovery screening tool. Further, compared to previously reported LC droplet biosensors, this LC droplet biosensor with a lipid coating is more biologically relevant and its ease of use in detecting membrane-related biological processes and interactions has the potential for development as a reliable, low-cost and disposable point of care diagnostic tool.

Keywords:	liquid crystal, microfluidics, lipids, droplets, anitimicrobial peptides
Subjects:	F000 - Physical sciences > F300 - Physics
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.1039/C8LC01291A Publication http://eprints.whiterose.ac.uk/141866/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	05 Mar 2019 18:39
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/498
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Bao, Peng https://orcid.org/0000-0002-7875-7420 University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Type of data: Dataset Research funders: EPSRC Grant numbers: EP/P024041/1 Publication date: 2019 Resource language: English Publisher: University of Leeds Contact Email Address: P.Bao@leeds.ac.uk Last Modified: 10 Jun 2020 17:11

Files

Documentation

Readme [156kB] Readme [156kB] Readme [156kB] Visible to: Anyone File name: Readme.pdf Content type: Documentation Mime-Type: application/pdf License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 156kB

Data

Fluoresence intensity vs channel number [32kB] Fluoresence intensity vs channel number [32kB] Fluoresence intensity vs channel number [32kB] Visible to: Anyone File name: Figure4b.xlsx Content type: Data Description: Original data for Fig4(b) Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 32kB

Droplet generation (slow dow of highspeed camera video) [13MB] Droplet generation (slow dow of highspeed camera video) [13MB] Droplet generation (slow dow of highspeed camera video) [13MB] Visible to: Anyone File name: figure_2d_video.avi Content type: Data Description: Original video for Fig2(d) Mime-Type: video/x-msvideo License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 13MB

Collection of images of droplets for size distribution analysis [1MB] Collection of images of droplets for size distribution analysis [1MB] Collection of images of droplets for size distribution analysis [1MB] Visible to: Anyone File name: 20171102-2timedilute_x10_size_and_concentration.jpg Content type: Data Description: Original image for Figure 5(C) Mime-Type: image/jpeg License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Switch of droplets in the flow of SMP43 in the trap structure [17MB] Switch of droplets in the flow of SMP43 in the trap structure [17MB] Switch of droplets in the flow of SMP43 in the trap structure [17MB] Visible to: Anyone File name: support_information_video2-SMP43_6uM_treatment_taken_every_5min.mov Content type: Data Description: Original video for Fig.6, Fig.7,and Fig.8 Mime-Type: video/quicktime License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 17MB

Size of droplets as a function of flow rate [33kB] Size of droplets as a function of flow rate [33kB] Size of droplets as a function of flow rate [33kB] Visible to: Anyone File name: Size-flowrateratio.xlsx Content type: Data Description: Original Data for Fig.S4 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 33kB

FRAP video taken for every 1s [5MB] FRAP video taken for every 1s [5MB] FRAP video taken for every 1s [5MB] Visible to: Anyone File name: every1s_frap.avi Content type: Data Description: Original video for Fig.S2 Mime-Type: video/x-msvideo License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 5MB