Research Data Leeds Repository

Dataset associated with 'Developing Hollow-channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes'

Ye, Sunjie and Wheeler, May C. and Mclaughlin, James R. and Tamang, Abiral and Diggle, Christine P. and Cespedes, Oscar and Markham, Alex F. and Coletta, P. Louise and Evans, Stephen D. (2018) Dataset associated with 'Developing Hollow-channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes'. University of Leeds. [Dataset] https://doi.org/10.5518/408

Dataset description

Gold nanoparticles-enabled intracellular surface-enhanced Raman spectroscopy (SERS) provides a sensitive and promising technique for single cell analysis. Compared with spherical gold nanoparticles, gold nanoflowers, i.e., flower-shaped gold nanostructures, can produce a stronger SERS signal. Current exploration of gold nanoflowers for intracellular SERS has been considerably limited by the difficulties in preparation, as well as background signal and cytotoxicity arising from the surfactant capping layer. Recently, we have developed a facile and surfactant-free method for fabricating hollow-channel gold nanoflowers (HAuNFs) with great single-particle SERS activity. In this paper, we investigate the cellular uptake and cytotoxicity of our HAuNFs using a RAW 264.7 macrophage cell line, and have observed effective cellular internalization and low cytotoxicity. We have further engineered our HAuNFs into SERS-active tags, and demonstrated the functionality of the obtained tags as trimodal nanoprobes for dark-field and fluorescence microscopy imaging, together with intracellular SERS.

Keywords:	gold nanoflowers; intracellular nanoprobes; surface plasmon resonance; SERS; fluorescence; dark-field
Subjects:	F000 - Physical sciences > F300 - Physics
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.3390/ijms19082327 Dataset http://eprints.whiterose.ac.uk/134767/ Dataset
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	06 Aug 2018 10:56
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/402
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Ye, Sunjie https://orcid.org/0000-0001-5152-5753 University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Wheeler, May C. Mclaughlin, James R. Tamang, Abiral University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Diggle, Christine P. https://orcid.org/0000-0001-6861-359X University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Cespedes, Oscar https://orcid.org/0000-0002-5249-9523 University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Markham, Alex F. University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Coletta, P. Louise University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Evans, Stephen D. https://orcid.org/0000-0001-8342-5335 University Of Leeds http://dx.doi.org/10.13039/501100000777 crossref Type of data: Dataset Publication date: 2018 Resource language: English Publisher: University of Leeds Contact Email Address: s.ye@leeds.ac.uk Last Modified: 20 Aug 2018 09:39

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Data

Results of spectral measurement [93kB] Results of spectral measurement [93kB] Results of spectral measurement [93kB] Visible to: Anyone File name: Figure2.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 93kB