Research Data Leeds Repository

Dataset associated with the publication 'Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks'

Corbett, S.L. and Sharma, Rajan and Davies, A Giles and Walti, Christoph (2017) Dataset associated with the publication 'Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks'. University of Leeds. [Dataset] https://doi.org/10.5518/136

Dataset description

Raw data of the experimental work reported in the publication entitled 'Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks’

Keywords:	RecA, nucleoprotein filaments, DNA
Subjects:	H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H163 - Bioelectronics & bioelectricity
Divisions:	Faculty of Engineering and Physical Sciences > School of Electronic and Electrical Engineering
Related resources:	Location Type https://doi.org/10.1038/srep41081 Publication http://eprints.whiterose.ac.uk/111207/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	18 Jan 2017 09:58
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/109
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Corbett, S.L. https://orcid.org/0000-0002-1242-1481 Sharma, Rajan https://orcid.org/0000-0003-1928-216X Davies, A Giles https://orcid.org/0000-0002-1987-4846 Walti, Christoph https://orcid.org/0000-0001-9286-5359 Type of data: Dataset Research funders: EPSRC Grant numbers: EP/J500124 Publication date: 2017 Resource language: English Publisher: University of Leeds Contact Email Address: c.walti@leeds.ac.uk Last Modified: 20 Mar 2017 12:08

Files

Data

AFM of fully-basepaired templates [353kB] AFM of fully-basepaired templates [353kB] AFM of fully-basepaired templates [353kB] Visible to: Anyone File name: AFM Fully-basepaired 10ng.001.tif Content type: Data Description: AFM image of fully basepaired templates (used for figure S2) Mime-Type: image/tiff File size: 353kB

AFM of fully-basepaired template (zoom-in) [186kB] AFM of fully-basepaired template (zoom-in) [186kB] AFM of fully-basepaired template (zoom-in) [186kB] Visible to: Anyone File name: AFM Fully-basepaired 10ng.002.tif Content type: Data Description: AFM image of fully basepaired template (used for figure 2) Mime-Type: image/tiff File size: 186kB

AFM of templates with mismatches [284kB] AFM of templates with mismatches [284kB] AFM of templates with mismatches [284kB] Visible to: Anyone File name: AFM 20bp-mismatch 25ng.001.tif Content type: Data Description: AFM image of basepair mismatched templates (used for figure S2) Mime-Type: image/tiff File size: 284kB

AFM of templates with mismatches (zoom-in) [60kB] AFM of templates with mismatches (zoom-in) [60kB] AFM of templates with mismatches (zoom-in) [60kB] Visible to: Anyone File name: AFM 20bp-mismatch 25ng.002.tif Content type: Data Description: AFM image of basepair mismatched template (used for figure 2) Mime-Type: image/tiff File size: 60kB

Proximal mismatches [2MB] Proximal mismatches [2MB] Proximal mismatches [2MB] Visible to: Anyone File name: Proximal mismatch (repeat 1).tif Content type: Data Description: PAGE image of restriction digest assay of promixal mismatches (used for figure 3) Mime-Type: image/tiff File size: 2MB

Proximal mismatches [2MB] Proximal mismatches [2MB] Proximal mismatches [2MB] Visible to: Anyone File name: Proximal mismatch (repeat 2).tif Content type: Data Description: PAGE image of restriction digest assay of promixal mismatches (used for figure 3) Mime-Type: image/tiff File size: 2MB

Proximal mismatches [2MB] Proximal mismatches [2MB] Proximal mismatches [2MB] Visible to: Anyone File name: Proximal mismatch (repeat 3).tif Content type: Data Description: PAGE image of restriction digest assay of promixal mismatches (used for figure 3) Mime-Type: image/tiff File size: 2MB

12bp mismatches [2MB] 12bp mismatches [2MB] 12bp mismatches [2MB] Visible to: Anyone File name: 12bp mismactch (repeat 1).tif Content type: Data Description: PAGE image of restriction digest assay of templates with 12bp mismatches at different positions (used for figure 4) Mime-Type: image/tiff File size: 2MB

12bp mismatches [2MB] 12bp mismatches [2MB] 12bp mismatches [2MB] Visible to: Anyone File name: 12bp mismactch (repeat 2).tif Content type: Data Description: PAGE image of restriction digest assay of templates with 12bp mismatches at different positions (used for figure 4) Mime-Type: image/tiff File size: 2MB

12bp mismatches [2MB] 12bp mismatches [2MB] 12bp mismatches [2MB] Visible to: Anyone File name: 12bp mismactch (repeat 3).tif Content type: Data Description: PAGE image of restriction digest assay of templates with 12bp mismatches at different positions (used for figure 4) Mime-Type: image/tiff File size: 2MB

Centered mismatches [2MB] Centered mismatches [2MB] Centered mismatches [2MB] Visible to: Anyone File name: Centered mismatches.tif Content type: Data Description: PAGE image of restriction digest assay of centred mismatches (used for figure 6) Mime-Type: image/tiff File size: 2MB

Centered mismatches [2MB] Centered mismatches [2MB] Centered mismatches [2MB] Visible to: Anyone File name: Centered mismatches (10 to 1).tif Content type: Data Description: PAGE image of restriction digest assay of centred mismatches using a ratio of filaments to template of 10:1 (used for figure S4) Mime-Type: image/tiff File size: 2MB

Centered mismatches [2MB] Centered mismatches [2MB] Centered mismatches [2MB] Visible to: Anyone File name: Centered mismatches (20 to 1).tif Content type: Data Description: PAGE image of restriction digest assay of centred mismatches using a ratio of filaments to template of 20:1 (used for figure S4) Mime-Type: image/tiff File size: 2MB

Nicked templates [2MB] Nicked templates [2MB] Nicked templates [2MB] Visible to: Anyone File name: Nicked templates (repeat 1).tif Content type: Data Description: PAGE image of restriction digest assay of templates with nicks at different locations (used for figure 6) Mime-Type: image/tiff File size: 2MB

Nicked templates [2MB] Nicked templates [2MB] Nicked templates [2MB] Visible to: Anyone File name: Nicked templates (repeat 2).tif Content type: Data Description: PAGE image of restriction digest assay of templates with nicks at different locations (used for figure 6) Mime-Type: image/tiff File size: 2MB

Nicked templates [2MB] Nicked templates [2MB] Nicked templates [2MB] Visible to: Anyone File name: Nicked templates (repeat 3).tif Content type: Data Description: PAGE image of restriction digest assay of templates with nicks at different locations (used for figure 6) Mime-Type: image/tiff File size: 2MB

Annealed templates [2MB] Annealed templates [2MB] Annealed templates [2MB] Visible to: Anyone File name: Annealed templates.tif Content type: Data Description: PAGE image showing all used templates Mime-Type: image/tiff File size: 2MB

Control [2MB] Control [2MB] Control [2MB] Visible to: Anyone File name: Complete digestion.tif Content type: Data Description: PAGE image showing complete digestion of template Mime-Type: image/tiff File size: 2MB

Control [2MB] Control [2MB] Control [2MB] Visible to: Anyone File name: No digestion.tif Content type: Data Description: PAGE image showing no digestion of template Mime-Type: image/tiff File size: 2MB