Research Data Leeds Repository

Mosquito saliva enhances virus infection through sialokinin-dependent vascular leakage

Lefteri, Daniella A. and Bryden, Steven and Pingen, Marieke and Terry, Sandra and McCafferty, Ailish and Beswick, Emily F and Georgiev, Georgi and Van der Laan, Marleen and Mastrullo, Valeria and Campagnolo, Paola and Waterhouse, Robert M and Varjak, Margus and Merits, Andres and Fragkoudis, Rennos and Griffin, Stephen and Shams, Kave and Pondeville, Emilie and McKimmie, Clive (2022) Mosquito saliva enhances virus infection through sialokinin-dependent vascular leakage. University of Leeds. [Dataset]

Dataset description

Viruses transmitted by Aedes mosquitoes are an increasingly important global cause of disease. Defining common determinants of host susceptibility to this large group of het- erogenous pathogens is key for informing the rational design of panviral medicines. Infection of the vertebrate host with these viruses is enhanced by mosquito saliva, a complex mixture of salivary-gland-derived factors and microbiota. We show that the enhancement of infection by saliva was dependent on vascular function and was inde- pendent of most antisaliva immune responses, including salivary microbiota. Instead, the Aedes gene product sialokinin mediated the enhancement of virus infection through a rapid reduction in endothelial barrier integrity. Sialokinin is unique within the insect world as having a vertebrate-like tachykinin sequence and is absent from nonvector competent Anopheles mosquitoes, whose saliva was not proviral and did not induce sim- ilar vascular permeability. Therapeutic strategies targeting sialokinin have the potential to limit disease severity following infection with Aedes-mosquito-borne viruses.

Keywords: mosquitoes ; arbovirus ; inflammation ; endothelium
Subjects: C000 - Biological sciences > C500 - Microbiology > C540 - Virology
Divisions: Faculty of Medicine and Health > School of Medicine
Related resources:
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 08 Jun 2022 13:32




Research Data Leeds Repository is powered by EPrints
Copyright © 2024 University of Leeds