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Citation

Lefteri, Daniella A. and Bryden, Steven and Pingen, Marieke and Terry, Sandra and McCafferty, Ailish and Beswick, Emily F. and Georgiev, Georgi and Van der Laan, Marleen and Mastrullo, Valeria and Campagnolo, Paola and Waterhouse, Robert M. and Varjak, Margus and Merits, Andres and Fragkoudis, Rennos and Griffin, Stephen and Shams, Kave and Pondeville, Emilie and McKimmie, Clive (2022) Mosquito saliva enhances virus infection through sialokinin-dependent vascular leakage. University of Leeds. [Dataset] https://doi.org/10.5518/1163

Dataset description

Viruses transmitted by Aedes mosquitoes are an increasingly important global cause of disease. Defining common determinants of host susceptibility to this large group of het- erogenous pathogens is key for informing the rational design of panviral medicines. Infection of the vertebrate host with these viruses is enhanced by mosquito saliva, a complex mixture of salivary-gland-derived factors and microbiota. We show that the enhancement of infection by saliva was dependent on vascular function and was inde- pendent of most antisaliva immune responses, including salivary microbiota. Instead, the Aedes gene product sialokinin mediated the enhancement of virus infection through a rapid reduction in endothelial barrier integrity. Sialokinin is unique within the insect world as having a vertebrate-like tachykinin sequence and is absent from nonvector competent Anopheles mosquitoes, whose saliva was not proviral and did not induce sim- ilar vascular permeability. Therapeutic strategies targeting sialokinin have the potential to limit disease severity following infection with Aedes-mosquito-borne viruses.

Keywords:	mosquitoes ; arbovirus ; inflammation ; endothelium
Subjects:	C000 - Biological sciences > C500 - Microbiology > C540 - Virology
Divisions:	Faculty of Medicine and Health > School of Medicine
Related resources:	Location Type https://eprints.whiterose.ac.uk/185862/ Publication https://doi.org/10.1073/pnas.2114309119 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	08 Jun 2022 13:32
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/974
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Lefteri, Daniella A. https://orcid.org/0000-0002-9985-4254 University of Leeds https://ror.org/024mrxd33 ror Bryden, Steven https://orcid.org/0000-0001-5408-6047 University of Leeds https://ror.org/024mrxd33 ror Pingen, Marieke https://orcid.org/0000-0001-5689-9076 University of Leeds https://ror.org/024mrxd33 ror Terry, Sandra University of Glasgow https://ror.org/00vtgdb53 ror McCafferty, Ailish https://orcid.org/0000-0002-4983-2014 University of Leeds https://ror.org/024mrxd33 ror Beswick, Emily F. https://orcid.org/0000-0002-4129-5891 University of Leeds https://ror.org/024mrxd33 ror Georgiev, Georgi University of Leeds https://ror.org/024mrxd33 ror Van der Laan, Marleen https://orcid.org/0000-0002-0754-4233 University of Leeds https://ror.org/024mrxd33 ror Mastrullo, Valeria https://orcid.org/0000-0002-6716-0370 University of Surrey https://ror.org/00ks66431 ror Campagnolo, Paola https://orcid.org/0000-0002-8957-3969 University of Surrey https://ror.org/00ks66431 ror Waterhouse, Robert M. https://orcid.org/0000-0003-4199-9052 University of Lausanne https://ror.org/019whta54 ror Varjak, Margus https://orcid.org/0000-0003-2608-5148 University of Glasgow https://ror.org/00vtgdb53 ror Merits, Andres https://orcid.org/0000-0001-8193-0071 University of Tartu https://ror.org/03z77qz90 ror Fragkoudis, Rennos https://orcid.org/0000-0002-8451-2665 University of Edinburgh https://ror.org/01nrxwf90 ror Griffin, Stephen https://orcid.org/0000-0002-7233-5243 University of Leeds https://ror.org/024mrxd33 ror Shams, Kave https://orcid.org/0000-0002-8145-5351 University of Leeds https://ror.org/024mrxd33 ror Pondeville, Emilie https://orcid.org/0000-0001-8545-8472 University of Glasgow https://ror.org/00vtgdb53 ror McKimmie, Clive https://orcid.org/0000-0002-7694-9509 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: MRC, Wellcome Trust Collection period: Date from Date to 1 October 2017 30 September 2020 Grant numbers: MC_UU_12014/8, 108227/B/15/Z Publication date: 2022 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: c.s.mckimmie@leeds.ac.uk Last Modified: 02 Sep 2024 10:30

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