This repository contains raw data associated with the publication: 'A comparative meta-proteomic
pipeline for the identification of plasmodesmata proteins and regulatory 
conditions in diverse plant species' -by Philip Kirk; Sam Amsbury; Liam German;
Rocio Gaudioso-Pedraza; YOSELIN BENITEZ-ALFONSO. BMC. Biology (2022)

Data is compressed into '.zip' files named according to which figure in the paper 
the data relates to. For more details on how the data was obtained please consult the Methods section in the publication.
Note: Raw data used for Figure 1-4 is provided within the supplementary files Additional File 1 and Additional File 2 published with the manuscript.
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Figure 5: GFP diffusion in the root meristem of Arabidopsis thaliana is restricted 
in 3% PEG and 75 mM sodium chloride. 

The data for this figure was collected from plants that were germinated 
directly on the treatment and grown for 4 days (subfolder 4dpg) or plants grown in control and transferred to the treatment 24 hours before data collection (subfolder 4dpg1dpt). The folder
structure within these subfolders is identical. Raw confocal images of root tips
are in the 'images' folder. These images are in'.czi' format that can be opened 
with Fiji imaging software. These images were used to generate fluorescence 
profiles across transects of the root (see publication for details). The transects
generated for the publication are stored in a '.csv' format within 'lateral 
analysis' or 'tz to tip analysis'. The column in the 'csv' named 'Value' 
is the mean fluorescence value (arbitrary units, AU) at a given point along the transect. How this
data is used is described in the methods section.
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Figure 6: SHR-GFP expression is reduced in the root meristem of Arabidopsis 
thaliana grown in 75 mM sodium chloride.

Data for this figure are derived from confocal images in a '.czi' format that can be 
opened with Fiji imaging software. This data is used to generate lateral 
fluorescent profiles (as described in Methods) as depicted in 
Figure 6a. The fluorescent profiles are stored in the folder 
'30 um from qc lateral analysis'. In a separate analysis, fluorescence was measured
for the endodermis, stele and QC using Fiji/ImageJ. The fluorescence readings are recorded
in 'endo vasc' (which related to Figure 6 d,f) and qc vasc (which relates to
Figure 6 e,g)
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Figure 7: The Medicago truncatula protein Medtr1g073320 is regulated in response 
to rhizobia and co-localizes with callose at plasmodesmata. 

This figure shows representative cropped confocal images. The raw confocal images
are in a '.czi' format and can be opened with Fiji imaging software.
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Figure 8: Ectopic Medtr1g073320 expression affects rhizobia infection and callose
 regulation in nitrate replete conditions.

Figure S9. Medtr1g073320 regulates rhizobia infection and nodulation in 
full-nitrate conditions.

These figures share the same dataset containing root phenotyping data presented here in Excel.
Column legend: Nitrate = nitrate treatment, cm = length of root in cm, IT = 
infection thread count, IT density = IT per cm of root, IP = infection pockets,
IP density = IP per cm of root, Nodules = nodules count, nodules density = nodules
per cm of root. 
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Figure S4.  Aniline blue staining reveals callose deposits in Arabidopsis root 
exposed to PEG and NaCl. 

This figure uses representative confocal images. The raw confocal images
are in a '.czi' format and can be opened with Fiji imaging software.
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Figure S5. Ectopic expression of the callose regulatory protein PDLP1 restricts 
root growth and response to 3% PEG. 

This folder contains scans of plates containing WT (wt) or PDLP1OE (LP1OE) seedlings
grown on either control (con), 0 mM nitrate (lowN), 0 mM K+ (lowK), 3% PEG (peg) or 75
mM NaCl (nacl) in '.tif' format. Rootnav was used to measure root morphology 
In these images. The spreadsheet 'formatted perplant .csv' contains the exported
data and is clearly annotated.
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Figure S6.  Medtr1g073320 is a PDLP- family member upregulated upon rhizobia
inoculation in Medicago truncatula roots. 

Spreadsheet with qPCR data obtained for the housekeeping gene actin and Medtr1g073320 (pdlp)
in a variety of treatments. CT values and data analysis to extract differential expression is shown.
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Figure S7.  Medtr1g073320 localizes with callose at plasmodesmata.

This figure shows representative confocal images. The raw confocal images
are in a '.czi' format and can be opened with Fiji imaging software.
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Figure S8. Medtr1g073320 overexpression improves root and shoot weight. 

Spreadsheet recording the root and shoot biomass (grams) of Medicago plants 
transformed with an empty vector (control) or with a construct
that lead to overexpression of Medtr1g073320. 
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