Research Data Leeds Repository

Citation

Anastasiou, Antonios and Jha, Animesh (2019) Antibacterial properties and regenerative potential of Sr2+ and Ce3+ doped fluorapatites; a potential solution for peri-implantitis. University of Leeds. [Dataset] https://doi.org/10.5518/717

Dataset description

Scaffolds and implants in orthopaedics and regenerative dentistry usually fail because of bacterial infections. A promising solution would be the development of biomaterials with both significant regenerative potential and enhanced antibacterial activity. Working towards this direction, fluorapatite was synthesised and doped with Sr2+ and Ce3+ ions in order to tailor its properties. After experiments with four common bacteria (i.e. E. Coli, S. Aureus, B. Subtilis, B. Cereus), it was found that the undoped and the Ce3+ doped fluorapatites present better antibacterial response than the Sr2+ doped material. The synthesised minerals were incorporated into chitosan scaffolds and tested with Dental Pulp Stem Cells (DPSCs) to check their regenerative potential. As was expected, the scaffolds containing Sr2+-doped fluorapatite, presented high osteoconductivity leading to the differentiation of the DPSCs into osteoblasts. Similar results were obtained for the Ce3+-doped material, since both the concentration of osteocalcin and the RUNX2 gene expression were considerably higher than that for the un-doped mineral. Overall, it was shown that doping with Ce3+ retains the good antibacterial profile of fluorapatite and enhances its regenerative potential, which makes it a promising option for dealing with conditions where healing of hard tissues is compromised by bacterial contamination.

Subjects:	A000 - Medicine & dentistry > A200 - Pre-clinical dentistry H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering
Divisions:	Faculty of Engineering and Physical Sciences > School of Chemical and Process Engineering
Related resources:	Location Type https://eprints.whiterose.ac.uk/152370/ Publication https://doi.org/10.1038/s41598-019-50916-4 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	14 Jan 2020 16:31
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/627
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Anastasiou, Antonios https://orcid.org/0000-0003-3858-9431 University of Leeds https://ror.org/024mrxd33 ror Jha, Animesh https://orcid.org/0000-0003-3150-5645 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Marie Curie, EPSRC Grant numbers: 660147, EP/N00941X/1 Publication date: 2019 Resource language: English Metadata language: English Research project title: PRe-Facto, Medical Technologies Innovation and Knowledge Centre Publisher: University of Leeds Last Modified: 30 Oct 2024 12:36

Files

Data

XRD data [146kB] XRD data [146kB] XRD data [146kB] Security: Anyone File name: XRD_fluorapatites.opj Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 146kB

FTIR data [415kB] FTIR data [415kB] FTIR data [415kB] Security: Anyone File name: Ftir_Data.opj Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 415kB

SEM images [659kB] SEM images [659kB] SEM images [659kB] Security: Anyone File name: SEM_images.pptx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.presentationml.presentation License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 659kB

IC50 and RUNX2 [36kB] IC50 and RUNX2 [36kB] IC50 and RUNX2 [36kB] Security: Anyone File name: IC50_and_RUNX2.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 36kB

Chitosan Scaffold EDX map [1MB] Chitosan Scaffold EDX map [1MB] Chitosan Scaffold EDX map [1MB] Security: Anyone File name: Chitosan_Scaffold_EDX_map_Site_29.docx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB