This readme.txt file was generated on 01.05.19 by Andrew Booth

-------------------
GENERAL INFORMATION
-------------------

Title of Dataset: Dataset associated with 'In vitro membrane remodelling by ESCRT-II/ESCRT-III is regulated by negative feedback from membrane tension'

Author Information:

Andrew Booth (1) a.booth@leeds.ac.uk
Christopher J. Marklew(2)
Barbara Ciani(2) Principle Investigator b.ciani@sheffield.ac.uk
Paul A. Beales(1) Principle Investigator p.a.beales@leeds.ac.uk

(1) School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
 
(2) Department of Chemistry and Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield, UK


Data collected 2017-18

Funding: Engineering and Physical Sciences Research Council (UK): EP/M027929/1 (Beales); EP/M027821/1 (Ciani)


--------------------------
SHARING/ACCESS INFORMATION
-------------------------- 

Licenses/restrictions placed on the data, or limitations of reuse: Creative Commons Attribution 4.0 International (CC BY 4.0)

Recommended citation for the data: Dataset associated with 'In vitro membrane remodelling by ESCRT-II/ESCRT-III is regulated by negative feedback from membrane tension'- cite as appropriate for the main publication in iScience, Cell Press.

Links to other publicly accessible locations of the data: http://review.researchdata.leeds.ac.uk/id/eprint/1189



--------------------
DATA & FILE OVERVIEW
--------------------

File list (filenames, directory structure (for zipped files) and brief description of all data files):

Name: 'Example_flickering_video_Figure_S2B.czi' (proprietry Zeiss filetype, may be opened in imageJ or the free Zeiss Zen software.
Descirption: Exemplar file of time course imaging of a GUV (Giant Unilamellar Vesicle) undergoing membrane undulations that was used for flicker analysis of the type described in the article.

Name: 'Histogram_data_Figures_S4_S5_S6.xlsx' Excel file
Descirption:Tabulated data and assosciated histogram plots for ILV number and size as seen in FIGURES S5, S5 and S6. 

Name: 'ILV_counting_data_statistical_analysis_Figures_3_4_and_5.opj' OriginPro file
Descirption:Tabulated data and assosciated plots for ILV number and size as seen in FIGURES 3, 4 and 5, plus statistical analysis by the ANOVA method. 

Name: 'Tension_boxplots_data_Figure_6_and_S2.opj' OriginPro file
Descirption:Tabulated data and assosciated plots for membrane tension values as described in Figures 6 and S2.


--------------------------
METHODOLOGICAL INFORMATION
--------------------------

Please refer to the iScience paper, 'Transparent Methods' section of the Supplementary Information.