This dataset contains electron microscopy imaging and experimental data as described in "https://pubs.acs.org/doi/10.1021/jacs.8b11480" cryoEM_images.zip contains all cryoEM images for CTB-CC2 as .png files DLS_Data.zip containes all the DLS data for CTB-CC2 and MBP-CC2 as .xlsx files negative_stain_EM_images_111115.zip contains the first set of negative stain EM images of CTB-CC2 as .tif files negative_stain_EM_images_250119.zip contains the second set of negative stain EM images or CTB-CC2 as .tif files PISA-interactions-all-penta-solvent.xlsx contains PISA data for CTB-CC SDS-PAGE_gels_for_CTBCC2_and_CC2a.zip contains all SDS-PAGE gels as .jpg files SEC_data.zip contains all SEC data for CTB-CC2, CTB-CC2a and MBP-CCX as .asc and .xlsx files