Research Data Leeds Repository

Lipid coated liquid crystal droplets for the on-chip detection of Antimicrobial peptides  - dataset

Citation

Bao, Peng (2019) Lipid coated liquid crystal droplets for the on-chip detection of Antimicrobial peptides  - dataset. University of Leeds. [Dataset] https://doi.org/10.5518/466¹

Dataset description

We describe a novel biosensor based on phospholipid-coated nematic liquid crystal (LC) droplets and demonstrate the detection of Smp43, a model antimicrobial peptide (AMP) from the venom of North African scorpion Scorpio maurus palmatus. Mono-disperse lipid-coated LC droplets of diameter 16.7 ± 0.2 μm were generated using PDMS microfluidic devices with a flow-focusing configuration and were the target for AMPs. The droplets were trapped in a bespoke microfluidic trap structure and were simultaneously treated with Smp43 at gradient concentrations in six different chambers. The disruption of the lipid monolayer by the Smp43 was detected (< 6 μM) at concentrations well within its biologically active range, indicated by a dramatic change in the appearance of the droplets associated with the transition from a typical radial configuration to a bipolar configuration, which is readily observed by polarizing microscopy. This suggests the system has feasibility as a drug-discovery screening tool. Further, compared to previously reported LC droplet biosensors, this LC droplet biosensor with a lipid coating is more biologically relevant and its ease of use in detecting membrane-related biological processes and interactions has the potential for development as a reliable, low-cost and disposable point of care diagnostic tool.

Keywords:	liquid crystal, microfluidics, lipids, droplets, anitimicrobial peptides
Subjects:	F000 - Physical sciences > F300 - Physics2
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy3
Related resources:	Location Type https://doi.org/10.1039/C8LC01291A4 Publication https://eprints.whiterose.ac.uk/141866/5 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	05 Mar 2019 18:39
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/4986
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Bao, Peng https://orcid.org/0000-0002-7875-74207 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: EPSRC Grant numbers: EP/P024041/1 Publication date: 2019 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: P.Bao@leeds.ac.uk8 Last Modified: 14 Oct 2024 09:42

Files

Documentation

Readme [156kB] 9 Readme [156kB] 10 Readme [156kB] 11 Security: Anyone File name: Readme.pdf12 Content type: Documentation Mime-Type: application/pdf License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 156kB

Data

Fluoresence intensity vs channel number [32kB] 13 Fluoresence intensity vs channel number [32kB] 14 Fluoresence intensity vs channel number [32kB] 15 Security: Anyone File name: Figure4b.xlsx16 Content type: Data Description: Original data for Fig4(b) Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 32kB

Droplet generation (slow dow of highspeed camera video) [13MB] 17 Droplet generation (slow dow of highspeed camera video) [13MB] 18 Droplet generation (slow dow of highspeed camera video) [13MB] 19 Security: Anyone File name: figure_2d_video.avi20 Content type: Data Description: Original video for Fig2(d) Mime-Type: video/x-msvideo License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 13MB

Collection of images of droplets for size distribution analysis [1MB] 21 Collection of images of droplets for size distribution analysis [1MB] 22 Collection of images of droplets for size distribution analysis [1MB] 23 Security: Anyone File name: 20171102-2timedilute_x10_size_and_concentration.jpg24 Content type: Data Description: Original image for Figure 5(C) Mime-Type: image/jpeg License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Switch of droplets in the flow of SMP43 in the trap structure [17MB] 25 Switch of droplets in the flow of SMP43 in the trap structure [17MB] 26 Switch of droplets in the flow of SMP43 in the trap structure [17MB] 27 Security: Anyone File name: support_information_video2-SMP43_6uM_treatment_taken_every_5min.mov28 Content type: Data Description: Original video for Fig.6, Fig.7,and Fig.8 Mime-Type: video/quicktime License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 17MB

Size of droplets as a function of flow rate [33kB] 29 Size of droplets as a function of flow rate [33kB] 30 Size of droplets as a function of flow rate [33kB] 31 Security: Anyone File name: Size-flowrateratio.xlsx32 Content type: Data Description: Original Data for Fig.S4 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 33kB

FRAP video taken for every 1s [5MB] 33 FRAP video taken for every 1s [5MB] 34 FRAP video taken for every 1s [5MB] 35 Security: Anyone File name: every1s_frap.avi36 Content type: Data Description: Original video for Fig.S2 Mime-Type: video/x-msvideo License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 5MB

• 1. https://doi.org/10.5518/466
• 2. https://archive.researchdata.leeds.ac.uk/view/subjects/F300.html
• 3. https://archive.researchdata.leeds.ac.uk/view/divisions/SPA/
• 4. https://doi.org/10.1039/C8LC01291A
• 5. https://eprints.whiterose.ac.uk/141866/
• 6. https://archive.researchdata.leeds.ac.uk/id/eprint/498
• 7. https://orcid.org/0000-0002-7875-7420
• 8. mailto:P.Bao@leeds.ac.uk
• 9. https://archive.researchdata.leeds.ac.uk/498/7/Readme.pdf
• 10. https://archive.researchdata.leeds.ac.uk/498/7/Readme.pdf
• 11. https://archive.researchdata.leeds.ac.uk/498/7/Readme.pdf
• 12. https://archive.researchdata.leeds.ac.uk/498/7/Readme.pdf
• 13. https://archive.researchdata.leeds.ac.uk/498/1/Figure4b.xlsx
• 14. https://archive.researchdata.leeds.ac.uk/498/1/Figure4b.xlsx
• 15. https://archive.researchdata.leeds.ac.uk/498/1/Figure4b.xlsx
• 16. https://archive.researchdata.leeds.ac.uk/498/1/Figure4b.xlsx
• 17. https://archive.researchdata.leeds.ac.uk/498/2/figure_2d_video.avi
• 18. https://archive.researchdata.leeds.ac.uk/498/2/figure_2d_video.avi
• 19. https://archive.researchdata.leeds.ac.uk/498/2/figure_2d_video.avi
• 20. https://archive.researchdata.leeds.ac.uk/498/2/figure_2d_video.avi
• 21. https://archive.researchdata.leeds.ac.uk/498/3/20171102-2timedilute_x10_size_and_concentration.jpg
• 22. https://archive.researchdata.leeds.ac.uk/498/3/20171102-2timedilute_x10_size_and_concentration.jpg
• 23. https://archive.researchdata.leeds.ac.uk/498/3/20171102-2timedilute_x10_size_and_concentration.jpg
• 24. https://archive.researchdata.leeds.ac.uk/498/3/20171102-2timedilute_x10_size_and_concentration.jpg
• 25. https://archive.researchdata.leeds.ac.uk/498/4/support_information_video2-SMP43_6uM_treatment_taken_every_5min.mov
• 26. https://archive.researchdata.leeds.ac.uk/498/4/support_information_video2-SMP43_6uM_treatment_taken_every_5min.mov
• 27. https://archive.researchdata.leeds.ac.uk/498/4/support_information_video2-SMP43_6uM_treatment_taken_every_5min.mov
• 28. https://archive.researchdata.leeds.ac.uk/498/4/support_information_video2-SMP43_6uM_treatment_taken_every_5min.mov
• 29. https://archive.researchdata.leeds.ac.uk/498/5/Size-flowrateratio.xlsx
• 30. https://archive.researchdata.leeds.ac.uk/498/5/Size-flowrateratio.xlsx
• 31. https://archive.researchdata.leeds.ac.uk/498/5/Size-flowrateratio.xlsx
• 32. https://archive.researchdata.leeds.ac.uk/498/5/Size-flowrateratio.xlsx
• 33. https://archive.researchdata.leeds.ac.uk/498/6/every1s_frap.avi
• 34. https://archive.researchdata.leeds.ac.uk/498/6/every1s_frap.avi
• 35. https://archive.researchdata.leeds.ac.uk/498/6/every1s_frap.avi
• 36. https://archive.researchdata.leeds.ac.uk/498/6/every1s_frap.avi