Research Data Leeds Repository

Citation

Armistead, Fern and Gala de Pablo, Julia and Gadêlha, Hermes and Peyman, Sally A. and Evans, Stephen D. (2019) Cells under stress: An inertial-shear microfluidic determination of cell behaviour - dataset. University of Leeds. [Dataset] https://doi.org/10.5518/397

Dataset description

The deformability of a cell is the direct result of a complex interplay between the different constituent elements at the subcellular level, coupling a wide range of mechanical responses at different length-scales. Changes to the structure of these components can also alter cell phenotype, thus the critical importance of cell mechano-response for diagnostic applications. The response to mechanical stress depends strongly on the forces experienced by the cell. Here we use cell deformability in both shear-dominant and inertia-dominant microfluidic flow regimes to probe different aspects of the cell structure. In the inertial regime we follow cellular response from (visco-)elastic through plastic deformation to cell structural failure and show a significant drop in cell viability for shear stresses above > 11.8 kN/m2. Comparatively, a shear-dominant regime requires lower applied stresses to achieve higher cell strains. From this regime, deformation traces as a function of time contain a rich source of information including; maximum strain, elastic modulus and cell relaxation times and thus provide a number of markers for distinguishing cell types and potentially disease progression. These results emphasise the benefit of multiple parameter determination for improving detection and will ultimately lead to improved accuracy for diagnosis. We present results for leukemia cells (HL60) as a model circulatory cell as well as for a colorectal cancer cell line SW480 derived from primary adenocarcinoma (Dukes stage B). SW480 were also treated with the actin disrupting drug Latrunculin A (LatA), to test the sensitivity of flow regimes to the cytoskeleton. We show that the shear regime is more sensitive to cytoskeletal changes, and that large strains in the inertial-regime cannot resolve changes to the actin cytoskeleton.

Subjects:	F000 - Physical sciences > F300 - Physics
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.1016/j.bpj.2019.01.034 Publication https://eprints.whiterose.ac.uk/142155/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	15 Feb 2019 19:02
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/487
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Armistead, Fern https://orcid.org/0000-0003-1274-5379 University of Leeds https://ror.org/024mrxd33 ror Gala de Pablo, Julia https://orcid.org/0000-0003-0557-9632 University of Leeds https://ror.org/024mrxd33 ror Gadêlha, Hermes https://orcid.org/0000-0001-8053-9249 University of York https://ror.org/04m01e293 ror Peyman, Sally A. https://orcid.org/0000-0002-1600-5100 University of Leeds https://ror.org/024mrxd33 ror Evans, Stephen D. https://orcid.org/0000-0001-8342-5335 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: EPSRC and MRC, MRC Grant numbers: EP/P023266/1, MR/M009084/1 Publication date: 2019 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: ed10fja@leeds.ac.uk Last Modified: 10 Oct 2024 13:59

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Data

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