Research Data Leeds Repository

Dataset associated with 'Developing Hollow-channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes'

Ye, Sunjie and Wheeler, May C. and Mclaughlin, James R. and Tamang, Abiral and Diggle, Christine P. and Cespedes, Oscar and Markham, Alex F. and Coletta, P. Louise and Evans, Stephen D. (2018) Dataset associated with 'Developing Hollow-channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes'. University of Leeds. [Dataset]

Dataset description

Gold nanoparticles-enabled intracellular surface-enhanced Raman spectroscopy (SERS) provides a sensitive and promising technique for single cell analysis. Compared with spherical gold nanoparticles, gold nanoflowers, i.e., flower-shaped gold nanostructures, can produce a stronger SERS signal. Current exploration of gold nanoflowers for intracellular SERS has been considerably limited by the difficulties in preparation, as well as background signal and cytotoxicity arising from the surfactant capping layer. Recently, we have developed a facile and surfactant-free method for fabricating hollow-channel gold nanoflowers (HAuNFs) with great single-particle SERS activity. In this paper, we investigate the cellular uptake and cytotoxicity of our HAuNFs using a RAW 264.7 macrophage cell line, and have observed effective cellular internalization and low cytotoxicity. We have further engineered our HAuNFs into SERS-active tags, and demonstrated the functionality of the obtained tags as trimodal nanoprobes for dark-field and fluorescence microscopy imaging, together with intracellular SERS.

Keywords: gold nanoflowers; intracellular nanoprobes; surface plasmon resonance; SERS; fluorescence; dark-field
Subjects: F000 - Physical sciences > F300 - Physics
Divisions: Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 06 Aug 2018 10:56




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