Research Data Leeds Repository

Citation

Ye, Sunjie and Wheeler, May C. and McLaughlan, James R. and Tamang, Abiral and Diggle, Christine P. and Cespedes, Oscar and Markham, Alexander F. and Coletta, P. Louise and Evans, Stephen D. (2018) Dataset associated with 'Developing Hollow-channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes'. University of Leeds. [Dataset] https://doi.org/10.5518/408

Dataset description

Gold nanoparticles-enabled intracellular surface-enhanced Raman spectroscopy (SERS) provides a sensitive and promising technique for single cell analysis. Compared with spherical gold nanoparticles, gold nanoflowers, i.e., flower-shaped gold nanostructures, can produce a stronger SERS signal. Current exploration of gold nanoflowers for intracellular SERS has been considerably limited by the difficulties in preparation, as well as background signal and cytotoxicity arising from the surfactant capping layer. Recently, we have developed a facile and surfactant-free method for fabricating hollow-channel gold nanoflowers (HAuNFs) with great single-particle SERS activity. In this paper, we investigate the cellular uptake and cytotoxicity of our HAuNFs using a RAW 264.7 macrophage cell line, and have observed effective cellular internalization and low cytotoxicity. We have further engineered our HAuNFs into SERS-active tags, and demonstrated the functionality of the obtained tags as trimodal nanoprobes for dark-field and fluorescence microscopy imaging, together with intracellular SERS.

Keywords:	gold nanoflowers; intracellular nanoprobes; surface plasmon resonance; SERS; fluorescence; dark-field
Subjects:	F000 - Physical sciences > F300 - Physics
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.3390/ijms19082327 Dataset http://eprints.whiterose.ac.uk/134767/ Dataset
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	06 Aug 2018 10:56
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/402
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Ye, Sunjie https://orcid.org/0000-0001-5152-5753 University of Leeds https://ror.org/024mrxd33 ror Wheeler, May C. University of Leeds https://ror.org/024mrxd33 ror McLaughlan, James R. https://orcid.org/0000-0001-5795-4372 University of Leeds https://ror.org/024mrxd33 ror Tamang, Abiral University of Leeds https://ror.org/024mrxd33 ror Diggle, Christine P. https://orcid.org/0000-0001-6861-359X University of Leeds https://ror.org/024mrxd33 ror Cespedes, Oscar https://orcid.org/0000-0002-5249-9523 University of Leeds https://ror.org/024mrxd33 ror Markham, Alexander F. https://orcid.org/0000-0002-6639-3199 University of Leeds https://ror.org/024mrxd33 ror Coletta, P. Louise https://orcid.org/0000-0002-3858-6073 University of Leeds https://ror.org/024mrxd33 ror Evans, Stephen D. https://orcid.org/0000-0001-8342-5335 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Publication date: 2018 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: s.ye@leeds.ac.uk Last Modified: 05 Aug 2024 08:02

Files

Documentation

ReadMe.txt [287B] ReadMe.txt [287B] ReadMe.txt [287B] Security: Anyone File name: ReadMe.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 287B

Data

Results of spectral measurement [93kB] Results of spectral measurement [93kB] Results of spectral measurement [93kB] Security: Anyone File name: Figure2.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 93kB