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Citation

Ferguson, Calum T. J. and Al-Khalaf, Areej A. and Isaac, R. Elwyn and Cayre, Olivier J. (2018) Data associated with 'pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii'. University of Leeds. [Dataset] https://doi.org/10.5518/398

Dataset description

Drosophila suzukii (or spotted wing Drosophila) is an economically important pest which can have a devastating impact on soft and stone fruit industries. Biological pesticides are being sought as alternatives to synthetic chemicals to control this invasive pest, but many are subject to degradation either in the environment or in the insect gut and as a result require protection. In this study we identified a sharp change in pH of the adult midgut from neutral to acidic (pH <3), which we then exploited to develop poly(2-vinylpyridine) ( P2VP) microcapsules that respond to the change in midgut pH by dissolution and release of their cargo for uptake into the insect. First, we used labelled solid poly(methyl methacrylate) (PMMA) particles to show that microcapsules with a diameter less than 15 μm are readily ingested by the adult insect. To encapsulate water-soluble biological species in an aqueous continuous phase, a multiple emulsion template was used as a precursor for the synthesis of pH-responsive P2VP microcapsules with a fluorescent (FITC-dextran) cargo. The water soluble agent was initially separated from the aqueous continuous phase by an oil barrier, which was subsequently polymerised. The P2VP microcapsules were stable at pH > 6, but underwent rapid dissolution at pH < 4.2. In vivo studies showed that the natural acidity of the midgut of D. suzukii also induced the breakdown of the responsive P2VP microcapsules to release FITC-dextran which was taken up into the body of the insect and accumulated in the renal tubules.

Keywords:	pH-triggered delivery; pesticide encapsulation; Drosophila Suzukii
Divisions:	Faculty of Biological Sciences > School of Biology Faculty of Engineering and Physical Sciences > School of Chemical and Process Engineering
Related resources:	Location Type https://doi.org/10.1371/journal.pone.0201294 Publication https://eprints.whiterose.ac.uk/134158/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	26 Jun 2018 08:28
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/380
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Ferguson, Calum T. J. https://orcid.org/0000-0002-6168-4624 University of Leeds https://ror.org/024mrxd33 ror Al-Khalaf, Areej A. https://orcid.org/0000-0001-9278-0822 University of Leeds https://ror.org/024mrxd33 ror Isaac, R. Elwyn https://orcid.org/0000-0003-4792-6559 University of Leeds https://ror.org/024mrxd33 ror Cayre, Olivier J. https://orcid.org/0000-0003-1339-3686 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Publication date: 2018 Resource language: English Metadata language: English Publisher: University of Leeds Last Modified: 05 Aug 2024 14:36

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