Research Data Leeds Repository

Dataset associated with 'Towards designer organelles by subverting the peroxisomal import pathway'.

Cross, Laura L. and Paudyal, Rupesh and Kamisugi, Yasuko and Berry, Alan and Cuming, Andrew C. and Baker, Alison and Warriner, Stuart L. (2017) Dataset associated with 'Towards designer organelles by subverting the peroxisomal import pathway'. University of Leeds. [Dataset]

Dataset description

This data is associated with a research project concerning development of tools to develop ‘designer’ organelles, a potential strategy to enable foreign pathways to be efficiently controlled within eukaryotic biotechnology. A fundamental component of any such system will be the implementation of a bespoke protein import pathway that can selectively deliver constituent proteins to the new compartment in the presence of existing endogenous trafficking systems. In the associated manuscript we show that the protein-protein interactions that control the peroxisomal protein import pathway can be manipulated to create a pair of interacting partners that still support protein import in moss cells but are orthogonal to the naturally occurring pathways. In addition to providing a valuable experimental tool to give new insights into peroxisomal protein import, the variant receptor-signal sequence pair forms the basis of a system in which normal peroxisomal function is downregulated and replaced with an alternative pathway, an essential first step in the creation of a designer organelle.

Subjects: F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry > F165 - Biomolecular chemistry
C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C750 - Plant biochemistry
Divisions: Faculty of Biological Sciences > School of Biology
Faculty of Biological Sciences > School of Molecular and Cellular Biology
Faculty of Engineering and Physical Sciences > School of Chemistry
Related resources:
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 06 Jul 2017 16:20




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