1. ABOUT THE DATASET -------------------- Title: Effect of microbubble-assisted gemcitabine delivery with repeated ultrasound exposure in a pancreatic cancer organ-on-a-chip model Creator(s): Delanyo Kpeglo [1], Malcolm Haddrick [2], Margaret A. Knowles [1], Stephen D. Evans [1], and Sally A. Peyman [3] Organisation(s): 1. University of Leeds. 2. Medicines Discovery Catapult. 3. Heriot-Watt University Rights-holder(s): Unless otherwise stated, Copyright 2022 University of Leeds Publication Year: 2025 Description: The data set contains raw data and images of results presented in the manuscript. It contains conceptual schematics, confocal images, and viability assessments of our pancreatic cancer organ-on-a-chip model with microbubble-assisted gemcitabine delivery with repeated ultrasound exposure. Cite as: Kpeglo et al (2025). Dataset for 'Effect of microbubble-assisted gemcitabine delivery with repeated ultrasound exposure in a pancreatic cancer organ-on-a-chip model'. University of Leeds. https://doi.org/10.5518/1218 2. TERMS OF USE --------------- Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.] 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: Biophysics of cancer: modelling and assessing physical resistance to therapy Dates: 2022-2024 Funding organisation: Engineering and Physical Sciences Research Council Grant no.: EP/P023266/1 4. CONTENTS ----------- File listing Dataset number 1: Figure_1_and_Figure_S1. This file contains a PowerPoint file of the schematic of the microbubble and US treatment of the on-chip PDAC culture, as well as a CAD schematic of the device used for the PDAC culture. Dataset number 2: Figure_2. This file contains bright-field images of the pancreatic cancer organ-on-a-chip model with increased culture time, along with an Excel file of the ATP viability assessment for the microfluidic PDAC culture. Dataset number 3: Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2. This file contains bright-field and fluorescence images and videos of the ATTO488 labelled microbubbles made, and the ATTO488 labelled microbubbles on-chip with the PDAC culture model or with BME gel only. Dataset number 4: Figure_4_and_Figure 5_and_Figure_6_and_Figure 7. The file contains a PowerPoint presentation illustrating a timeline of the seeding of PDAC cells into our microfluidic device for developing our pancreatic cancer organ-on-a-chip model. It includes the treatment of our model with microbubbles, gemcitabine, and ultrasound exposure, an Excel file with the ATP viability assessment of our model after treatment as well as that of a 2D culture of the PDAC cells (control) with the same treatments, and confocal images along with their quantification of our PDAC culture model subjected to microbubbles, gemcitabine, and repeated ultrasound exposure treatment. 5. METHODS ---------- - Experimental procedures/protocols are as described in the manuscript. - AutoDesk's AutoCAD was used to design the microfluidic device, which was made using photolithography, and from which moulds were produced using soft lithography. See the manuscript for details. - Microbubbles were produced in-house using microfluidics, and ultrasound exposure was conducted either once or repeatedly, as described in the manuscript. - Confocal images were obtained using a Leica TCS SP8 confocal laser scanning microscope, as outlined in the manuscript. - Viability was assessed by quantifying the ATP content of our model. We performed 2D culture of the PDAC cells, treating them with microbubbles, gemcitabine, and ultrasound as a control for our model. These details are described in the manuscript.