1. ABOUT THE DATASET -------------------- Title: Dataset for Nanomachine Networks: Functional All-Enzyme Hydrogels from Photochemical Cross-Linking of Glucose Oxidase Creator(s): Harrison Laurent [1], David Brockwell [2], Lorna Dougan [1,2] Organisation(s): 1. School of Physics and Astronomy, University of Leeds, Leeds, UK, LS2 9JT. 2. Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK, LS2 9JT Rights-holder(s):Unless otherwise stated, Copyright 2024 University of Leeds Publication Year: 2025 Description: Data associated with the publication of Nanomachine Networks: Functional All-Enzyme Hydrogels from Photochemical Cross-Linking of Glucose Oxidase. Raw data is presented as a series of .csv files, descriptions of which can be found in data_deposit_Laurent_H_DOI_1590.xlsx. These files then produce the published data by running the python scripts within the data repository, descriptions of which are also found in data_deposit_Laurent_H_DOI_1590.xlsx. Cite as: 'Nanomachine Networks: Functional All-Enzyme Hydrogels from Photochemical Cross-Linking of Glucose Oxidase'. University of Leeds. [Dataset] https://doi.org/10.5518/1590. Related publication: Laurent, H.; Brockwell, D. J.; Dougan, L. Nanomachine Networks: Functional All-Enzyme Hydrogels from Photochemical Cross-Linking of Glucose Oxidase. Biomacromolecules 2025, Accepted. Contact: L.Dougan@leeds.ac.uk 2. TERMS OF USE --------------- [A standard copyright notice and licence statement with URL can be used, e.g. Copyright [publication year] [University of Leeds, name of other rights-holder(s)]. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.] 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: MESONET: Exploiting in situ protein unfolding to understand and control mesoscopic network formation Dates: 1/9/22 to 31/8/27 Funding organisation: ERC and UKRI Grant no.: EP/X023524/1 4. CONTENTS ----------- File listing All raw data files are contained within the zip file, raw_data.zip, and python scripts are contained within the zip file, python_scripts.zip. Descriptions of the files and file names of .csv files containing raw data, and python scripts used to read in raw data files and plot data within the zip file for associated publication are listed below: Time resolved storage and loss moduli of GluOx hydrogels - GluOx conc sweep FULL 26062024.csv Freqency sweeps of GluOx hydrogels - GluOx conc sweep freq sweep FULL 26062024 Circular dichroism spectra for 50 mg/mL GluOx pregel solution - repeat 1 - GluOx_pregel_220824.csv Circular dichroism spectra for 50 mg/mL GluOx pregel solution - repeat 2 - GluOx_50mgmL_pregel_used_12122024.csv Circular dichroism spectra for 90 mg/mL GluOx pregel solution - repeat 1 - GluOx_pregel_used.csv Circular dichroism spectra for 90 mg/mL GluOx pregel solution - repeat 2 - GluOx_pregel00006_used.csv Circular dichroism spectra for 50 mg/mL GluOx gel - repeat 1 - GluOx_gel_overnight_220824.csv Circular dichroism spectra for 50 mg/mL GluOx gel - repeat 2 - GluOx_50mgmL_overnight_12122024.csv Circular dichroism spectra for 90 mg/mL GluOx gel - repeat 1 - GluOx_gel_overnight.csv Circular dichroism spectra for 90 mg/mL GluOx gel - repeat 2 - GluOx_gel00001.csv Raw data for Trinder assay on free enzyme - repeat 1 - GluOx free enzyme kinetics 1 15082024.csv Raw data for Trinder assay on free enzyme - repeat 2 - GluOx free enzyme kinetics 2 16082024.csv Raw data for Trinder assay on free enzyme - repeat 3 - GluOx free enzyme kinetics 3 21082024.csv Raw data for Trinder assay on gelled enzyme - repeat 1 - GluOx gel enzyme kinetics 1 15082024.csv Raw data for Trinder assay on gelled enzyme - repeat 2 - GluOx gel enzyme kinetics 2 21082024.csv Raw data for Trinder assay on gelled enzyme - repeat 3 - GluOx gel enyzme kinetics 3 21082024.csv Summary document for the values generated from the raw rheology data found in manuscript - Rheology_plotted_values.csv Python script to read in raw rheology data files and perform analysis - GluOx conc sweep FULL plotter.py Python script to read in raw Trinder assay data files and perform analysis - Trinder_assay_15082024.py Python script to read in raw CD data files and perform analysis - CD overnight plotter.py Python script to model hydrogel enzyme activity - Fickian_modelling_real_SUBMIT.py 5. METHODS ---------- Full methods are given in Materials and Methods section of main text, as well as in the associated Supplementary Information