1. ABOUT THE DATASET -------------------- Title: mRNAseq to investigate the immunological effects of intrahepatic artery delivery of TG6002 in combination with oral 5-fluorocytosine. Creator(s): Emma West, Adel Samson Organisation(s): University of Leeds. Rights-holder(s): Copyright 2024 University of Leeds Publication Year: 2024 Description: A phase 1 study of TG6002 vaccinia virus infusion via the hepatic artery in combination with oral 5-flurocytosine to colorectal cancer patients with liver metastases. Patient PBMCs were isolated from peripheral blood samples taken throughout treatment. mRNA sequencing was performed to investigate the immune response to combination treatment. Differential gene expression analysis revealed an association with viral dose; cluster analysis highlighted several pathways relevant to immune responses, type I interferon signalling and specific response to virus. Cite as: West, Emma and Samson, Adel (2024) Dataset for 'mRNAseq to investigate the immunological effects of intrahepatic artery delivery of TG6002 in combination with oral 5-fluorocytosine'. University of Leeds. [Dataset] https://doi.org/10.5518/1593. Related publication: Emma J. West, Alain Sadoun, Kaidre Bendjama, Philippe Erbs, Cristina Smolenschi, Philippe A. Cassier, Thierry de Baere, Sophie Sainte-Croix, Maud Brandely, Alan A. Melcher, Fay Ismail, Karen J. Scott, Angela Bennett, Emma Banks, Ewa Gasior, Sarah Kent, Marta Kurzawa, Christopher Hammond, Jai V. Patel, Fiona J. Collinson, Chris Twelves, D. Alan Anthoney, Dan Swinson, Adel Samson. A phase I clinical trial of intrahepatic artery delivery of TG6002 in combination with oral 5-fluorocytosine in patients with liver-dominant metastatic colorectal cancer. Clinical Cancer Research, 2024; accepted. Contact: a.samson@leeds.ac.uk 2. TERMS OF USE --------------- [A standard copyright notice and licence statement with URL can be used, e.g. Copyright [publication year] [University of Leeds, name of other rights-holder(s)]. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.] 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: mRNAseq to investigate the immunological effects of intrahepatic artery delivery of TG6002 in combination with oral 5-fluorocytosine. Dates: October 2019-April 2023 Funding organisation: CRUK Grant no.: 29039 4. CONTENTS ----------- File listing Fastq files are available for six sets of patient samples, each consisting of 8 files. Individual patients are identified as 'TG' with a numerical suffix, e.g. 01, 005, etc. The time point of samples are depicted by codes; SC, screening; BS, baseline; C1D2, cycle 1 day 2; C1D4, cycle 1 day 4; C1D15, cycle 1 day 15; C1E, cycle 1 day 2; C1F, cycle 1 day 4; C1H, cycle 1 day 15. TG01_C1E_1.fq.gz TG01_C1E_2.fq.gz TG01_C1F_1.fq.gz TG01_C1F_2.fq.gz TG01_C1H_1.fq.gz TG01_C1H_2.fq.gz TG01_SC_1.fq.gz TG01_SC_2.fq.gz TG03_C1E_1.fq.gz TG03_C1E_2.fq.gz TG03_C1F_1.fq.gz TG03_C1F_2.fq.gz TG03_C1H_1.fq.gz TG03_C1H_2.fq.gz TG03_SC_1.fq.gz TG03_SC_2.fq.gz TG04_C1E_1.fq.gz TG04_C1E_2.fq.gz TG04_C1F_1.fq.gz TG04_C1F_2.fq.gz TG04_C1H_1.fq.gz TG04_C1H_2.fq.gz TG04_SC_1.fq.gz TG04_SC_2.fq.gz TG_005_BS_1.fq.gz TG_005_BS_2.fq.gz TG_005_C1D2_1.fq.gz TG_005_C1D2_2.fq.gz TG_005_C1D4_1.fq.gz TG_005_C1D4_2.fq.gz TG_005_C1D15_1.fq.gz TG_005_C1D15_2.fq.gz TG_007_BS_1.fq.gz TG_007_BS_2.fq.gz TG_007_C1D2_1.fq.gz TG_007_C1D2_2.fq.gz TG_007_C1D4_1.fq.gz TG_007_C1D4_2.fq.gz TG_007_C1D15_1.fq.gz TG_007_C1D15_2.fq.gz TG_007_BS_1.fq.gz TG_008_BS_2.fq.gz TG_008_C1D2_1.fq.gz TG_008_C1D2_2.fq.gz TG_008_C1D4_1.fq.gz TG_008_C1D4_2.fq.gz TG_008_C1D15_1.fq.gz TG_008_C1D15_2.fq.gz 5. METHODS ---------- Detailed information about methods are available in the paper due to be published, details in section 1.