Research Data Leeds Repository

Citation

Fenn, Katherine and Horne, Jim E. and Crossley, Joel A. and Calabrese, Antonio N. and Radford, Sheena E. and Ranson, Neil A. (2024) Data for 'Outer membrane protein assembly mediated by BAM-SurA complexes'. University of Leeds. [Dataset] https://doi.org/10.5518/1460

Dataset description

The outer membrane is a formidable barrier that protects Gram-negative bacteria against environmental threats. Its integrity requires the correct folding and insertion of outer membrane proteins (OMPs) by the membrane-embedded β-barrel assembly machinery (BAM). Unfolded OMPs are delivered to BAM by the periplasmic chaperone SurA, but how SurA and BAM work together to ensure successful OMP delivery and folding remains unclear. Here, we present a dataset of single-molecule FRET of SurA and its interaction with the BAM alongside two alphafold predictions of the formed complex.

Keywords:	BAM, SurA, OMP, smFRET, Cryo-EM, alphafold
Subjects:	C000 - Biological sciences
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology Faculty of Biological Sciences > School of Molecular and Cellular Biology
Related resources:	Location Type https://doi.org/10.1038/s41467-024-51358-x Publication https://eprints.whiterose.ac.uk/216160/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	04 Sep 2024 09:53
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1320
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Fenn, Katherine https://orcid.org/0000-0003-0638-2241 University of Leeds https://ror.org/024mrxd33 ror Horne, Jim E. https://orcid.org/0000-0001-5260-2634 University of Leeds https://ror.org/024mrxd33 ror Crossley, Joel A. https://orcid.org/0000-0002-6656-1578 University of Leeds https://ror.org/024mrxd33 ror Calabrese, Antonio N. https://orcid.org/0000-0003-2437-7761 University of Leeds https://ror.org/024mrxd33 ror Radford, Sheena E. https://orcid.org/0000-0002-3079-8039 University of Leeds https://ror.org/024mrxd33 ror Ranson, Neil A. https://orcid.org/0000-0002-3640-5275 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: MRC, BBSRC, Wellcome, Royal Society, BMBF, DZIF Grant numbers: MR/P018491/1, BB/X015653/1, BB/T008059/1, 108466/Z/15/Z, 221524/Z/20/Z, 223810/Z/21/Z, RSRP/R1/211057, (220628/Z/20/Z, GBi2S, 9.918 Publication date: 2024 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: j.a.crossley@leeds.ac.uk Last Modified: 04 Sep 2024 11:25

Files

Documentation

readme1460.txt [4kB] readme1460.txt [4kB] readme1460.txt [4kB] Security: Anyone File name: readme1460.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 4kB

Data

SurA_Core-PPIase1.hdf5 [424MB] SurA_Core-PPIase1.hdf5 [424MB] SurA_Core-PPIase1.hdf5 [424MB] Security: Anyone File name: SurA_Core-PPIase1.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 424MB

SurA_Core-PPIase1_BAM.hdf5 [458MB] SurA_Core-PPIase1_BAM.hdf5 [458MB] SurA_Core-PPIase1_BAM.hdf5 [458MB] Security: Anyone File name: SurA_Core-PPIase1_BAM.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 458MB

SurA_Core-PPIase1_WEYIPNV.hdf5 [199MB] SurA_Core-PPIase1_WEYIPNV.hdf5 [199MB] SurA_Core-PPIase1_WEYIPNV.hdf5 [199MB] Security: Anyone File name: SurA_Core-PPIase1_WEYIPNV.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 199MB

SurA_Core-PPIase1_WEYIPNV_BAM.hdf5 [529MB] SurA_Core-PPIase1_WEYIPNV_BAM.hdf5 [529MB] SurA_Core-PPIase1_WEYIPNV_BAM.hdf5 [529MB] Security: Anyone File name: SurA_Core-PPIase1_WEYIPNV_BAM.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 529MB

SurA_Core-PPIase2.hdf5 [166MB] SurA_Core-PPIase2.hdf5 [166MB] SurA_Core-PPIase2.hdf5 [166MB] Security: Anyone File name: SurA_Core-PPIase2.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 166MB

SurA_Core-PPIase2_BAM.hdf5 [497MB] SurA_Core-PPIase2_BAM.hdf5 [497MB] SurA_Core-PPIase2_BAM.hdf5 [497MB] Security: Anyone File name: SurA_Core-PPIase2_BAM.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 497MB

SurA_Core-PPIase2_WEYIPNV.hdf5 [240MB] SurA_Core-PPIase2_WEYIPNV.hdf5 [240MB] SurA_Core-PPIase2_WEYIPNV.hdf5 [240MB] Security: Anyone File name: SurA_Core-PPIase2_WEYIPNV.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 240MB

SurA_Core-PPIase2_WEYIPNV_BAM.hdf5 [508MB] SurA_Core-PPIase2_WEYIPNV_BAM.hdf5 [508MB] SurA_Core-PPIase2_WEYIPNV_BAM.hdf5 [508MB] Security: Anyone File name: SurA_Core-PPIase2_WEYIPNV_BAM.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 508MB

BAM_SurA_AlphaFold2Multimer.pdb [1MB] BAM_SurA_AlphaFold2Multimer.pdb [1MB] BAM_SurA_AlphaFold2Multimer.pdb [1MB] Security: Anyone File name: BAM_SurA_AlphaFold2Multimer.pdb Content type: Data Mime-Type: application/vnd.palm License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

SurA_POTRA1-5_ColabFoldPrediction.pdb [514kB] SurA_POTRA1-5_ColabFoldPrediction.pdb [514kB] SurA_POTRA1-5_ColabFoldPrediction.pdb [514kB] Security: Anyone File name: SurA_POTRA1-5_ColabFoldPrediction.pdb Content type: Data Mime-Type: application/vnd.palm License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 514kB