Research Data Leeds Repository

Data for 'Dual client binding sites in the ATP-independent chaperone SurA'

Citation

Schiffrin, Bob and Crossley, Joel A. (2024) Data for 'Dual client binding sites in the ATP-independent chaperone SurA'. University of Leeds. [Dataset] https://doi.org/10.5518/1423¹

Dataset description

The ATP-independent chaperone SurA protects unfolded outer membrane proteins (OMPs) from aggregation in the periplasm of Gram-negative bacteria and delivers them to the beta-barrel assembly machinery (BAM) for folding into the outer membrane (OM). Precisely how SurA recognises and binds its different OMP clients remains unclear. E. coli SurA comprises three domains: a core and two PPIase domains (P1 and P2). Here, we present single-molecule Förster resonance energy transfer (smFRET) data showing conformational changes of unfolded OmpX (apo-OmpX.hdf5) when bound to SurA wild-type (OmpX+SurA-WT.hdf5), the SurA core domain (OmpX+SurA-core.hdf5) and finally, in 4 M urea (apo-OmpX_4M_Urea.hdf5).

Keywords:	NMR, smFRET, SurA, OmpX, OMP, chaperones.
Subjects:	C000 - Biological sciences2
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology3 Faculty of Biological Sciences > School of Molecular and Cellular Biology4
Related resources:	Location Type https://doi.org/10.1038/s41467-024-52021-15 Publication https://eprints.whiterose.ac.uk/216546/6 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	06 Aug 2024 16:00
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/13107
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Schiffrin, Bob https://orcid.org/0000-0002-1670-73568 University of Leeds https://ror.org/024mrxd33 ror Crossley, Joel A. https://orcid.org/0000-0002-6656-15789 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: BBSRC, Wellcome, Royal Society, EPSRC, European Regional Development Fund-Project, Czech Science Foundation Grant numbers: BB/T000635/1, 094232/Z/10/Z, 105615/Z/14/Z, 094232/Z/10/Z, 220628/Z/20/Z, RSRP/R1/211057, BB/T008059/1, BB/V003577/1, 222373/Z/21/Z, EP/N013573/1, BB/V003577/1, CZ.02.1.01/0.0/0.0/15_003/0000441, 20-11563Y Publication date: 2024 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: j.a.crossley@leeds.ac.uk10 Last Modified: 03 Dec 2024 12:25

Files

Documentation

readme_Crossley_2024.txt [3kB] 11 readme_Crossley_2024.txt [3kB] 12 readme_Crossley_2024.txt [3kB] 13 Security: Anyone File name: readme_Crossley_2024.txt14 Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3kB

Data

apo-OmpX.hdf5 [351MB] 15 apo-OmpX.hdf5 [351MB] 16 apo-OmpX.hdf5 [351MB] 17 Security: Anyone File name: apo-OmpX.hdf518 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 351MB

apo-OmpX_4M_Urea.hdf5 [115MB] 19 apo-OmpX_4M_Urea.hdf5 [115MB] 20 apo-OmpX_4M_Urea.hdf5 [115MB] 21 Security: Anyone File name: apo-OmpX_4M_Urea.hdf522 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 115MB

OmpX-SurA-core.hdf5 [862MB] 23 OmpX-SurA-core.hdf5 [862MB] 24 OmpX-SurA-core.hdf5 [862MB] 25 Security: Anyone File name: OmpX-SurA-core.hdf526 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 862MB

OmpX-SurA-WT.hdf5 [948MB] 27 OmpX-SurA-WT.hdf5 [948MB] 28 OmpX-SurA-WT.hdf5 [948MB] 29 Security: Anyone File name: OmpX-SurA-WT.hdf530 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 948MB

• 1. https://doi.org/10.5518/1423
• 2. https://archive.researchdata.leeds.ac.uk/view/subjects/C000.html
• 3. https://archive.researchdata.leeds.ac.uk/view/divisions/ACSMB/
• 4. https://archive.researchdata.leeds.ac.uk/view/divisions/SMCB/
• 5. https://doi.org/10.1038/s41467-024-52021-1
• 6. https://eprints.whiterose.ac.uk/216546/
• 7. https://archive.researchdata.leeds.ac.uk/id/eprint/1310
• 8. https://orcid.org/0000-0002-1670-7356
• 9. https://orcid.org/0000-0002-6656-1578
• 10. mailto:j.a.crossley@leeds.ac.uk
• 11. https://archive.researchdata.leeds.ac.uk/1310/5/readme_Crossley_2024.txt
• 12. https://archive.researchdata.leeds.ac.uk/1310/5/readme_Crossley_2024.txt
• 13. https://archive.researchdata.leeds.ac.uk/1310/5/readme_Crossley_2024.txt
• 14. https://archive.researchdata.leeds.ac.uk/1310/5/readme_Crossley_2024.txt
• 15. https://archive.researchdata.leeds.ac.uk/1310/1/apo-OmpX.hdf5
• 16. https://archive.researchdata.leeds.ac.uk/1310/1/apo-OmpX.hdf5
• 17. https://archive.researchdata.leeds.ac.uk/1310/1/apo-OmpX.hdf5
• 18. https://archive.researchdata.leeds.ac.uk/1310/1/apo-OmpX.hdf5
• 19. https://archive.researchdata.leeds.ac.uk/1310/2/apo-OmpX_4M_Urea.hdf5
• 20. https://archive.researchdata.leeds.ac.uk/1310/2/apo-OmpX_4M_Urea.hdf5
• 21. https://archive.researchdata.leeds.ac.uk/1310/2/apo-OmpX_4M_Urea.hdf5
• 22. https://archive.researchdata.leeds.ac.uk/1310/2/apo-OmpX_4M_Urea.hdf5
• 23. https://archive.researchdata.leeds.ac.uk/1310/3/OmpX-SurA-core.hdf5
• 24. https://archive.researchdata.leeds.ac.uk/1310/3/OmpX-SurA-core.hdf5
• 25. https://archive.researchdata.leeds.ac.uk/1310/3/OmpX-SurA-core.hdf5
• 26. https://archive.researchdata.leeds.ac.uk/1310/3/OmpX-SurA-core.hdf5
• 27. https://archive.researchdata.leeds.ac.uk/1310/4/OmpX-SurA-WT.hdf5
• 28. https://archive.researchdata.leeds.ac.uk/1310/4/OmpX-SurA-WT.hdf5
• 29. https://archive.researchdata.leeds.ac.uk/1310/4/OmpX-SurA-WT.hdf5
• 30. https://archive.researchdata.leeds.ac.uk/1310/4/OmpX-SurA-WT.hdf5