Research Data Leeds Repository

Citation

Schiffrin, Bob and Crossley, Joel A. (2024) Data for 'Dual client binding sites in the ATP-independent chaperone SurA'. University of Leeds. [Dataset] https://doi.org/10.5518/1423

Dataset description

The ATP-independent chaperone SurA protects unfolded outer membrane proteins (OMPs) from aggregation in the periplasm of Gram-negative bacteria and delivers them to the beta-barrel assembly machinery (BAM) for folding into the outer membrane (OM). Precisely how SurA recognises and binds its different OMP clients remains unclear. E. coli SurA comprises three domains: a core and two PPIase domains (P1 and P2). Here, we present single-molecule Förster resonance energy transfer (smFRET) data showing conformational changes of unfolded OmpX (apo-OmpX.hdf5) when bound to SurA wild-type (OmpX+SurA-WT.hdf5), the SurA core domain (OmpX+SurA-core.hdf5) and finally, in 4 M urea (apo-OmpX_4M_Urea.hdf5).

Keywords:	NMR, smFRET, SurA, OmpX, OMP, chaperones.
Subjects:	C000 - Biological sciences
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology Faculty of Biological Sciences > School of Molecular and Cellular Biology
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	06 Aug 2024 16:00
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1310
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Schiffrin, Bob https://orcid.org/0000-0002-1670-7356 University of Leeds https://ror.org/024mrxd33 ror Crossley, Joel A. https://orcid.org/0000-0002-6656-1578 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: BBSRC, Wellcome, Royal Society, EPSRC, European Regional Development Fund-Project, Czech Science Foundation Grant numbers: BB/T000635/1, 094232/Z/10/Z, 105615/Z/14/Z, 094232/Z/10/Z, 220628/Z/20/Z, RSRP/R1/211057, BB/T008059/1, BB/V003577/1, 222373/Z/21/Z, EP/N013573/1, BB/V003577/1, CZ.02.1.01/0.0/0.0/15_003/0000441, 20-11563Y Publication date: 2024 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: j.a.crossley@leeds.ac.uk Last Modified: 15 Aug 2024 14:48

Files

Documentation

readme_Crossley_2024.txt [3kB] readme_Crossley_2024.txt [3kB] readme_Crossley_2024.txt [3kB] Security: Anyone File name: readme_Crossley_2024.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3kB

Data

apo-OmpX.hdf5 [351MB] apo-OmpX.hdf5 [351MB] apo-OmpX.hdf5 [351MB] Security: Anyone File name: apo-OmpX.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 351MB

apo-OmpX_4M_Urea.hdf5 [115MB] apo-OmpX_4M_Urea.hdf5 [115MB] apo-OmpX_4M_Urea.hdf5 [115MB] Security: Anyone File name: apo-OmpX_4M_Urea.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 115MB

OmpX-SurA-core.hdf5 [862MB] OmpX-SurA-core.hdf5 [862MB] OmpX-SurA-core.hdf5 [862MB] Security: Anyone File name: OmpX-SurA-core.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 862MB

OmpX-SurA-WT.hdf5 [948MB] OmpX-SurA-WT.hdf5 [948MB] OmpX-SurA-WT.hdf5 [948MB] Security: Anyone File name: OmpX-SurA-WT.hdf5 Content type: Data License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 948MB