1. ABOUT THE DATASET -------------------- Title: Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension [Dataset] Creator(s): Benjamin J. Lane [1], Yue Ma [2], Nana Yan [1], Bolin Wang [2], Katrin Ackermann [3], Theodoros K. Karamanos [4], Bela E. Bode [3] and Christos Pliotas [1,2,5] Organisation(s): 1. Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, United Kingdom 2. School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic and Health Science Centre, The University of Manchester, Manchester, M13 9PT, United Kingdom 3. EaStCHEM School of Chemistry, Biomedical Sciences Research Complex and Centre of Magnetic Resonance, University of St Andrews, St Andrews, KY16 9ST, United Kingdom 4. Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, SW7 2AZ, United Kingdom 5. Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, United Kingdom Rights-holder(s): Benjamin J. Lane, Yue Ma, Nana Yan, Bolin Wang, Katrin Ackermann, Theodoros K. Karamanos, Bela E. Bode, Christos Pliotas, University of Leeds, University of St Andrews, University of Manchester. Publication Year: 2024 Description: The dataset contains the experimental NMR and EPR data that underpins the figures in the associated manuscript. Cite as: Benjamin J. Lane, Yue Ma, Nana Yan, Bolin Wang, Katrin Ackermann, Theodoros K. Karamanos, Bela E. Bode and Christos Pliotas (2024) Dataset for 'Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension'. University of Leeds. [Dataset] https://doi.org/10.5518/1267. Related publication: Benjamin J. Lane, Yue Ma, Nana Yan, Bolin Wang, Katrin Ackermann, Theodoros K. Karamanos, Bela E. Bode and Christos Pliotas. (2024). Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension'. Structure. Contact: christos.pliotas@manchester.ac.uk 2. TERMS OF USE --------------- Copyright 2024 Benjamin J. Lane, Yue Ma, Nana Yan, Bolin Wang, Katrin Ackermann, Theodoros K. Karamanos, Bela E. Bode, Christos Pliotas, University of Leeds, University of St Andrews, University of Manchester. This dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.]. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Funding organisations: BBSRC, Wellcome, Leverhulme Trust Grant no.: BB/S018069/1, 219999/Z/19/Z, 223268/Z/21/Z, RPG-2018-397, BB/R013780/1, BB/T017740/1. 4. CONTENTS ----------- The dataset contains all the raw and processed EPR spectroscopy data that underpins the figures. It also contains experimental data that underpins the NMR figures. Additionally, it contains the associated code required for extracting peak positions from the raw data and for the fitting of peak positions and intensities. A full file listing for the EPR data can be found in the EPR_Data folder called 'Underpinning_EPR_Data.pdf'. A description of the NMR data files can be found in the 'README.txt' in the NMR_Data folder. 5. METHODS ---------- Full details of the methods can be found in the STAR Methods section of the associated article. Benjamin J. Lane, Yue Ma, Nana Yan, Bolin Wang, Katrin Ackermann, Theodoros K. Karamanos, Bela E. Bode and Christos Pliotas. (2024). Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension'. Structure.