Title:	On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression
Creator(s): Alisha Farooq[1, 2], Christopher D. Wood [3],  John E. Ladbury [2],  Stephen D. Evans [1]

Organisation(s): [1] School of Physics and Astronomy, University of Leeds; [2] School of Molecular and Cellular Biology, University of Leeds; 
[3] School of Electronic and Electrical Engineering, University of Leeds

Rights-holder: Alisha Farooq, Chris Wood, John Ladbury, Stephen Evans.  

Publication Year: 2023

Description: Within the DOI folder there are 4 folders:(1) .mat and .py scripts (labelled in order of analysis) which contains the 
relevant MATLAB and python scripts requried for data processing and analysis. (2) analysed datasets (.mat and .csv) which contains the
post-emsc (.mat) and pre-processing (.csv) analysis dataset files for cell lines HET-1A, CP-A, CP-B, CP-C, CP-D & OE19.
(3) figures (.tif) which contains the paper figures as well as the supplementary figure. (4) raw datasets (.mat) which contains datamatrix raw files
for cell lines HET-1A, CP-A, CP-B, CP-C, CP-D & OE19 as well as the PDMS background and Biological reference files required for EMSC. 

Cite as: Farooq, Wood, Ladbury, Evans (2023). Dataset for 'On-chip Raman spectroscopy of live single cells for the staging of oesophageal 
adenocarcinoma progression'. University of Leeds. https://doi.org/10.5518/1246. 

Contact: primary author bsafa@leeds.ac.uk. corresponding author S.D.Evans@leeds.ac.uk


CONTENTS
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- Folder 1 (.mat and .py scripts). There is a note provided in the folder listing the function of each script and the order of data analysis.
 Each script is sufficiently annotated to provide information re each step and any editing requrired by future users. 

- Folder 2 (analysed datasets). For each dataset there is a .mat and a .csv file which can be immediately imported into Matlab or MS Excel software.
 Each file is named with the relevant cell line. .mat files haev undergone emsc analysis. Variables saved within the .mat file include "DataMatrix" with the EMSC-corrected data,
"CalibrationPeakTemp" contains the measured silicon peak position for that day, later on used to correct the spectra, "Error" which contains the standard 
error, "Average" for the average Y values across all the analysed spectra and "XValuesTrunc" which contains the new X values post-spline. All .csv files 
have undergone Raman pre-processing steps post-EMSC background correction. Within the .csv file there are headings for "Wavenumber", "Data(spectranumber)",
"mean" and "SD1".

- Folder 3 (figures (.tif)) contains all the relevant figures for the paper including the supplementary figure.

- Folder 4(raw datasets (.mat)). This folder contains the datasetraw. files for for cell lines HET-1A, CP-A, CP-B, CP-C, CP-D & OE19 as well as the PDMS 
background and Biological reference files required for EMSC. These files were generated using raw .wdf files and complied using script1 from Folder 1.