1. ABOUT THE DATASET -------------------- Title: Dataset associated with "Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study" Creator(s): Raelene M Cowie, Louise M Jennings Organisation(s): Institute of Medical and Biological Engineering, School of Mechanical Engineering, University of Leeds Rights-holder(s):Unless otherwise stated, Copyright 2024 University of Leeds Publication Year: 2024 Description: Dataset to support the publication "Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study". The dataset contains figures to support the methods, particularly the analysis techniques used includinng a detailed description of the cartilage grading methods, additional detaiols relating to measurement of the wear scar depth as well as details relating to the preparation of the grafts and the kinematic inputs used. Raw data from scoring of the cartilage surfaces has also been included. Cite as: Cowie RM & Jennings LM (2024): Dataset associated with "Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study". University of Leeds. [Dataset] https://doi.org/10.5518/1406 Related publication: Cowie RM, Macri-Pellizzeri L, McLaren J, Sanderson WJ, Felfel R, Scotchford CA, Scammell BE, Grant DM, Sottile V, Jennings LM. (2024) Functional performance of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold: a pre-clinical in vitro tribological study, Royal Society Open Science (Accepted) https://doi.org/10.1098/rsos.230431 Contact: r.cowie@leeds.ac.uk; l.m.jennings@leeds.ac.uk 2. TERMS OF USE --------------- Copyright 2024 University of Leeds. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.] 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: Preclinical functional performance assessment of a new bio-inspired non-delaminating osteochondral construct using a physiological knee implantation model Funding organisation: EPSRC; Versus Arthritis; Italian Ministry of University and Research Grant no.: EP/N00941X/1; 21501 4. CONTENTS ----------- File listing cartilage_grading.pdf Figures to show the methods used to assess wear, damage and deformation using cartilage grading wear_depth.pdf Method of measurement of wear scar depth from Accutrans replicas measured on an Alicona G5 optical profiler scaffold_preparation.pdf Details relating to scaffold production simulator_inputs.pdf Input profiles to simulator representative of a walking gait, scaled for use with porcine tissue cartilage_grading_score.pdf Cartilage grading score for meniscus, tibia and femur after 3 hours wear simulation 5. METHODS ---------- Full experimental methods are provided in the paper and supplementary data. The study involved 20 porcine knee joints which were dissected and set up for testing in a knee simulator as described by liu et al 2015. The input kinematics represented a walking gait cycle and were derived from Leeds high kinematic conditions (McEwen 2005) and adapted for use in the porcine knee joint as described by Bowland 2018 and Liu 2019. An osteochondral intervention was implanted in the medical condyle of each knee and consisted of either a chitosan osteochondral construct (OCC), a predicate device in current clinical use or a stainless steel pin implanted proud of the articulating surfaces which acted as a positive control. The influence of surgical positioning of the OCC, either inverted or proud was also assessed. The knees were run for 3 hours in a lubricant of 25% bovine serum in Ringers solution with the intervention in situ. At the conclusion of the study, the articulating surfaces of the joint were visually scored (see supplementary figures) to determine the wear, damage and deformaiton of the joint. Micro-CT scanning of the graft and surrounding bone was also carried out post-test. For the positive control the area and depth of the wear scar in the meniscus was assessed, the method used was insufficiently sensitive to analyse the other experimental groups.