1. ABOUT THE DATASET -------------------- Title: Substitution of Met-38 to Ile in γ-synuclein found in two patients with amyotrophic lateral sclerosis induces aggregation into amyloid Creator(s): Liam D. Aubrey,a, Natalia Ninkinab,c, Sabine M. Ulamec,a, Natalia Y. Abramycheva,d, Eftychia Vasili,e, Oliver M. Devine,a, Martin Wilkinson,a, Eilish Mackinnon,b, Galina Limorenko,b,e, Martin Walko,a,f, Sarah Muwanga,b, Leonardo Amadio,b, Owen M. Peters,b, Sergey N. Illarioshkin,d, Tiago F. Outeiro,g,h,i,j, Neil A. Ranson,a, David J. Brockwell,a, Vladimir L. Buchman,b,c, and Sheena E. Radford,a Organisation(s): a Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Science, University of Leeds, Leeds LS2 9JT, United Kingdom; b School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom; c Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University, Belgorod 308015, Russian Federation; d Laboratory of Neurobiology and Tissue Engineering, Brain Science Institute, Research Center of Neurology, Moscow 125367, Russia; e Laboratory of Molecular and Chemical Biology of Neurodegeneration, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne CH- 1015, Switzerland; f Astbury Centre for Structural Molecular Biology, School of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom; g Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Göttingen 37075, Germany; h Max Planck Institute for Multidisciplinary Sciences, Goettingen 37075, Germany; i Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle Upon Tyne NE2 4HH, United Kingdom;j Scientific employee with a honorary contract at Deutsches Zentrum für Neurodegenerative Erkrankungen, Göttingen 37075, Germany Rights-holder(s): Copyright 2024 University of Leeds Publication Year: 2024 Description: A collection of the biophysical data presented in the associated publication. Cite as: Liam D Aubrey, Sabine M. Ulamec, Natalia Ninkina, Natalia Y. Abramycheva, Galina Limorenko, Eftychia Vasili, Oliver M. Devine, Martin Wilkinson, Martin Walko, Tiago F. Outeiro, Kirill D. Chaprov, Sarah Muwanga, Leonardo Amadio, Owen M. Peters, Sergey N. Illarioshkin, Neil A Ranson, David J. Brockwell, Vladimir L. Buchman, Sheena E. Radford (2023): A newly discovered ALS-linked variant of γ-synuclein induces aggregation into amyloid. [Dataset]. https://doi.org/10.5518/1315 Related publication: L. D. Aubrey, et al., Substitution of Met-38 to Ile in γ-synuclein found in two patients with amyotrophic lateral sclerosis induces aggregation into amyloid. Proc. Natl. Acad. Sci. 121, e2309700120 (2024). 2. TERMS OF USE --------------- Copyright 2024 University of Leeds. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- High- performance liquid chromatography was funded by Wellcome (204963). All Electron Microscopy was performed at the Astbury Biostructure Laboratory, funded by the University of Leeds and Wellcome (221524/Z/20/Z). S.E.R. holds a Royal Society Research Professorship (RSRP/R1/211057), which also funds L.D.A. S.M.U. was funded by Wellcome (215062/Z/18/Z), and M. Wilkinson is funded by the Medical Research Council UK (MR/T011149/1). Circular Dichroism was funded by Wellcome (094232). We thank Professor Andy Wilson for help with peptide synthesis funded by Engineering and Physical Sciences research council (EP/N013573/1) and Biotechnology and Biological Sciences research council (BB/V003577/1). M. Walko is funded by the BBSRC (BB/T011157/1). T.F.O. is sup-ported by the Deutsche Forschung gemeinschaft (German Research Foundation) under Germany’s Excellence Strategy—EXC 2067/1- 390729940, by SFB1286 (B8). The study was also supported by a Grant from the Ministry of Science and Higher Education of RF (agreement No. 075- 15- 2021- 1346 to V.L.B.). Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. pJFRC5- 5XUAS- IVS- mCD8::GFP was a gift from Gerald Rubin, Addgene plasmid # 26218. O.M.P. is funded by the Medical Research Council (MC/PC/16030/2) and receives support from the UK Dementia Research Institute (DRI), which receives its funding from DRI Ltd., funded by the UK Medical Research Council, Alzheimer’s Society, and Alzheimer’s Research UK 4. CONTENTS ----------- File listing CD_data - circular dichroism data - supplementary figure S8 Differential pelleting - Quantification of the pelletable material by SDS-PAGE at the ThT endpoints - figures 2e and 5c Liposome ThT data - ThT data collected in the presence of liposomes - supplementary figure S8 Percentage soluble HPLC - Quantification of pelletable material at the ThT endpoints by HPLC - figure 1e Beads and Shaking ThTs - ThT data collected in the presence of beads with shaking - figures 1d, 2a, 5a Percent of transfected neuroblastoma cells - Quantification of the number of transfected neuroblastoma cells - figure 4b Seeded after beads and shaking ThT - ThT data collected quiescently - supplementary figure S4