1. ABOUT THE DATASET -------------------- Title: Biology Exploits Geometry Impact of Aspect Ratio on Protein Networks Creator(s): Matt D G Hughes [1], Sophie Cussons [2,3], Benjamin S Hanson [1], Kalila R Cook [1], Tímea Feller [4], Najet Mahmoudi [5], Daniel Baker [1], Robert Ariëns [4], David A Head [6], David J Brockwell [2,3], Lorna Dougan* [1,2] Organisation(s): [1] School of Physics and Astronomy, Faculty of Engineering and Physical Sciences, University of Leeds, UK [2] Astbury Centre for Structural Molecular Biology, University of Leeds, UK [3] School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, UK [4] School of Medicine, Faulty of Medicine, University of Leeds, UK [5] ISIS Neutron and Muon Spallation Source, STFC Rutherford Appleton Laboratory, Oxfordshire, UK [6] School of Computing, Faculty of Engineering and Physical Science, University of Leeds, UK Rights-holder(s):Unless otherwise stated, Copyright 2023 University of Leeds Publication Year: 2023 Description: This repository contains the raw data files and access to the code used to Cite as: Hughes et al. (2023):Biology Exploits Geometry Impact of Aspect Ratio on Protein Networks. University of Leeds. https://doi.org/10.5518/1344 Related publication: Hughes et al. Biology Exploits Geometry Impact of Aspect Ratio on Protein Networks. Nat. Comms. Accepted (2023) Contact: L.Dougan@leeds.ac.uk 2. TERMS OF USE --------------- Copyright University of Leeds, 2023. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: Exploiting Engineered Polyproteins in the Modular Design of Robust, Tuneable and Biofunctional Hydrogels Dates: 01 March 2018 - 31 January 2024 Funding organisation: EPSRC Grant no.: EP/ P02288X/1 4. CONTENTS ----------- All data is split between 2 folders named: Experimental Data & Simulation Data, which contain the raw data from experiment methods and simulation methods, respectively. Experimental Data folder contains all experimental data broken into subfolders by technique shown below: Rheology Contains subfolders of all rheology data as xlsx files broken down by the protien mesaured i.e. Fibrinogen and pL_[x], where x is the number of domains in the polyprotein chain. Scattering Contains all scattering data further broken down into subfolders below: SANS Contains txt files of SANS data for each protien L contruct. Files are saved in the format pL[x]_[concentration in mg/ml], where x is the number of domains in the polyprotien chain SAXS Contains a txt file of the SAXS curve shown in SI figure S2 DLS Contains folders of each concentration of pL_7 tested. The txt file meta data contains all relevant infomation such as measured angle. Circular Dichroism Contains xlsx files of CD data for each protien L contruct. Files are saved in the format pL[x]_[concentration in mg/ml], where x is the number of domains in the polyprotien chain Simulation Data BioNet Contains all raw data produced by the BioNet simulations with accompanying ReadMe file. Kinetic Lattice Model Contains all raw data produced by the kinetic lattice model simulations with accompanying ReadMe file. Finally there is a folder labeled code accessiblity which contains infomation on how to access the code of both simulations. 5. METHODS ---------- Experimental and simulation methods are outlined in the main manuscript and supplementary infomation.