1. ABOUT THE DATASET
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Title: An engineered Escherichia coli community for studying quorum sensing data set	

Creator(s): Yuwei Li, Justin Clarke, Alex O’Neil, Francisco M. Goycoolea, and James Smith

Organisation(s): University of Leeds

Rights-holder(s): Copyright 2023 University of Leeds

Publication Year: 2023

Description: The dataset contains (i) a file of designed plasmid sequences and the corresponding sequencing results and (ii) a file of raw data generated using a microplate reader, fluorometer, or spectrophotometer and the corresponding calculated data generated using MS Excel.

Cite as: Yuwei Li, Justin Clarke, Alex O’Neil, Francisco M. Goycoolea, and James Smith (2023) Dataset for 'An engineered Escherichia coli community for studying quorum sensing.' University of Leeds. [Dataset]  https://doi.org/10.5518/1341.

Related publication: Li, Y.W., Clarke, J., O'Neil, A., Goycoolea, F.M., Smith, J. An engineered Escherichia coli community for studying quorum sensing. Submitted: SynBio - 23/04/2023

Contact: F.M.Goycoolea@leeds.ac.uk

2. TERMS OF USE
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Copyright [2023] [University of Leeds]. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.

3. PROJECT AND FUNDING INFORMATION
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Title: [title of project]

Dates: [project start-end]

Funding organisation: Leeds Biofilm Network and School of Food Science and Nutrition, Faculty of Environment

Grant no.:

4. CONTENTS
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The dataset includes two files. 
(i) Plasmid_sequences.xlsx: designed plasmid sequences for three bacterial strains (sensor, producer, and regulator strains) and the corresponding whole plasmid sequencing results. 
(ii) Experimental_data.xlsx: raw experimental data (fluorescence intensity (FI) and optical density (OD600)), and calculated data (background subtracted or normalised FI by OD600) for plots generated using MS Excel. Different experimental conditions, e.g., the presence or absence of the inducer arabinose or sample supernatants, to test culture induction, taken at different time points, indicated in the different sheets. Different strains are in the different sheets.

5. METHODS
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For the detailed experimental procedure and protocol used to generate the dataset, refer to 'Section 4. Materials and Methods' of the article.