1. ABOUT THE DATASET -------------------- Title: On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan–DNA Polyelectrolyte Complexes Data Set Creator(s): Ayesha Sajid, Matteo Castronovo, Francisco M. Goycoolea [1] Organisation(s):[1] University of Leeds Rights-holder(s): Copyright 2023 University of Leeds Publication Year:2023 Description: The dataset contains all the results and raw data obtained from different characterisation techniques (AF4, nsTEM, SEC, UV Spectrophotometry, Zetasizer) and data underlying the figures included in the research article ' On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan–DNA Polyelectrolyte Complexes'. Cite as: Sajid, Ayesha., Castronovo, Matteo, and Goycoolea, Francisco (2023) Dataset for 'On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan–DNA Polyelectrolyte Complexes'. University of Leeds. [Dataset] https://doi.org/10.5518/1340. Related publication: Sajid, A.; Castronovo, M.; Goycoolea, F.M. On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan–DNA Polyelectrolyte Complexes. Polymers 2023, 15(9), 2115; https://doi.org/10.3390/polym15092115. Contact: F.M.Goycoolea@leeds.ac.uk 2. TERMS OF USE --------------- Copyright [2023] [University of Leeds]. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: [title of project] Dates: [project start-end] Funding organisation: Commonwealth Scholarship Commission, PKCS-2019-493 Grant no.: 4. CONTENTS ----------- The dataset includes five different folders, named on the experimental technique used to characterise either the different chitosan samples or their polyelectrolyte complexes with calf thymus DNA. -AF4:The directory comprises of two subfolders. 'AF4 Mw Analysis' folder contains the file 'AF4-Average molar mass distribution results.xlsx' containing average molecular weight distributions of NAS-032 and NAS-075 and their derivatives (Table 3).The folder also contains the MALS and RI results along with the molar masses of depolymerised chitosan (file name: AF4- Depoly nas-032 and depoly nas-075.xlsx) and their parent chitosan (file name: AF4-Parent Chitosan NAS-032 and NAS-075.xlsx) determined by asymmetric flow field-flow fractionation (AF4). Data presented in Figure 3. The 'AF4 PEC analysis' folder contains file 'AF4 data for depoly chitosan PEC.xlsx' with MALS and UV signal of polyplexes formed with depolymerised chitosan oligomers at N/P charge ratio 20 at the cross-flow of 3mL/min along with the average radii (Rg and Rh) and shape factor values of these PECs.The data is presented in Figure 8, 10, 11 and S6. The folder also contains the Rg values of all replicates of parent chitosan-DNA polyelectrolyte complexes at N/P 5, 10 and 20, along with the MALS 90°, UV and Rh data of the 3rd replicate, at cross-flow= 1mL/min in file 'AF4 data for PEC, XF 1mL min.xlsx' and at 3 mL/min in file 'AF4 data for PEC, XF 3mL min.xlsx'. The MALS 90° signal is presented in Figure 5 and for Rg, Rh and UV signal refer to Figure 6, 7 and S5. The 'Rg, Rh and shape factor of PEC.xlsx' file contains the summarised average values of Rg determined through AF4, hydrodynamic diameter (d) and radius (Rh) determined through online DLS and shape factor (Rg/Rh) of PECs comprising parent chitosan at different ratios and cross-flow rates. Averaged values are presented in Figure 10 and 11. -nsTEM: This folder includes six additional folders containing tif images of chitosan(NAS032 and NAS075)-DNA polyelectrolytec complexes at N/P 5, 10 and 20 obtained by negative staining transmission electron microscopy (Figure 12). -SEC: folder includes file 'SEC-Average molar mass distribution results.xlsx' with the average molecular wight distributions of parent chitosan and their depolymerised detrivatives obtained by SEC (Table 3). The folder also contains MALS 90° and RI signal of depolymerised and parent chitosan in 'SEC-Depoly nas-032 and depoly nas-075.xlsx' and 'SEC-Parent chitosan NAS-032 and NAS-075.xlsx' files respectively along with their molar mass, Rg and concentration values obtained from size exclusion chromatography. The concentration was determined through RI detector. Data shown in Figure 2, S1 and S6. -UV Spectrophotometry:The 'DA of Chitosan.xlsx' file in this folder contains the UV absorbance values and caliberation curve of the standard GlcNAc recorded at 201 nm used to determine the DA of NAS-032 and NAS-075.(Table 2) -Zetasizer Ultra:Comprised of two subfolders. The subfolder titled 'Size (depoly nas032 and depoly nas075 PEC)' contains the average hydrodynamic diameter and radius (Rh) values of polyelectrolyte complexes formed by depolymerised nas-032 and depoly nas-075 at N/P charge ratios (0.1, 0.5, 1, 2, 5, 10 and 20) determined by batch mode non-invasive backscattering dynamic light scattering (refer to file ' Average batch mode DLS results.xlsx' and Figure 9).The files 'NIBS-DLS, DNA-depoly nas-032 PEC.xlsx' and 'NIBS-DLS, DNA-depoly nas-075 PEC.xlsx' contains the raw data of hydrodynamic diameter (d.nm), intensity and correlation co-efficient measurements of PECs formed by depoly nas-032 and depoly nas-075 chitosan, respectively, at various N/P charge ratios. The subfolder named 'Zeta potential' contains average zeta potential values of polyplexes formed by different types of chitosan at multiple charge ratios in file 'Average zeta potential results.xlsx'. The raw zeta potential measurements of PECs at varying N/P charge ratios obatined from zetasizer are included in files 'Zeta potential DNA-depoly nas032.xlsx', 'Zeta potential DNA-depoly nas075.xlsx', 'Zeta potential DNA-NAS032.xlsx' and 'Zeta potential DNA-NAS075.xlsx' corresponding to each type of chitosan used to form the PECs (Figure 4). Abbreviations - AF4: Asymmetric flow field-flow fractionation - DLS: Dynamic light scattering - GlcNAc: N- acetyl Glucosamine - MALS 90°: Multiangle light scattering at 90 degree angle - PEC: Polyelectrolyte complex - RI: Refractive index - Rh: Hydrodynamic radius - Rg: Radius of gyration - ROI: Region of interest - SEC: Size exclusion chromatography - nsTEM: Negative staining transmission electron microscopy 5. METHODS ---------- For the detailed experimental procedure and protocol used to generate the dataset, refer to 'Section 2. Materials and Methods' of the article.