Research Data Leeds Repository

Dataset associated with the publication 'Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks'

Citation

Corbett, Sybilla L. and Sharma, Rajan and Davies, A. Giles and Wälti, Christoph (2017) Dataset associated with the publication 'Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks'. University of Leeds. [Dataset] https://doi.org/10.5518/136¹

Dataset description

Raw data of the experimental work reported in the publication entitled 'Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks’

Keywords:	RecA, nucleoprotein filaments, DNA
Subjects:	H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H163 - Bioelectronics & bioelectricity2
Divisions:	Faculty of Engineering and Physical Sciences > School of Electronic and Electrical Engineering3
Related resources:	Location Type https://doi.org/10.1038/srep410814 Publication https://eprints.whiterose.ac.uk/111207/5 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	18 Jan 2017 09:58
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1096
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Corbett, Sybilla L. https://orcid.org/0000-0002-1242-14817 University of Leeds https://ror.org/024mrxd33 ror Sharma, Rajan https://orcid.org/0000-0003-1928-216X8 University of Leeds https://ror.org/024mrxd33 ror Davies, A. Giles https://orcid.org/0000-0002-1987-48469 University of Leeds https://ror.org/024mrxd33 ror Wälti, Christoph https://orcid.org/0000-0001-9286-535910 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: EPSRC Grant numbers: EP/J500124 Publication date: 2017 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: c.walti@leeds.ac.uk11 Last Modified: 21 Aug 2024 08:18

Files

Data

AFM of fully-basepaired templates [353kB] 12 AFM of fully-basepaired templates [353kB] 13 AFM of fully-basepaired templates [353kB] 14 Security: Anyone File name: AFM Fully-basepaired 10ng.001.tif15 Content type: Data Description: AFM image of fully basepaired templates (used for figure S2) Mime-Type: image/tiff File size: 353kB

AFM of fully-basepaired template (zoom-in) [186kB] 16 AFM of fully-basepaired template (zoom-in) [186kB] 17 AFM of fully-basepaired template (zoom-in) [186kB] 18 Security: Anyone File name: AFM Fully-basepaired 10ng.002.tif19 Content type: Data Description: AFM image of fully basepaired template (used for figure 2) Mime-Type: image/tiff File size: 186kB

AFM of templates with mismatches [284kB] 20 AFM of templates with mismatches [284kB] 21 AFM of templates with mismatches [284kB] 22 Security: Anyone File name: AFM 20bp-mismatch 25ng.001.tif23 Content type: Data Description: AFM image of basepair mismatched templates (used for figure S2) Mime-Type: image/tiff File size: 284kB

AFM of templates with mismatches (zoom-in) [60kB] 24 AFM of templates with mismatches (zoom-in) [60kB] 25 AFM of templates with mismatches (zoom-in) [60kB] 26 Security: Anyone File name: AFM 20bp-mismatch 25ng.002.tif27 Content type: Data Description: AFM image of basepair mismatched template (used for figure 2) Mime-Type: image/tiff File size: 60kB

Proximal mismatches [2MB] 28 Proximal mismatches [2MB] 29 Proximal mismatches [2MB] 30 Security: Anyone File name: Proximal mismatch (repeat 1).tif31 Content type: Data Description: PAGE image of restriction digest assay of promixal mismatches (used for figure 3) Mime-Type: image/tiff File size: 2MB

Proximal mismatches [2MB] 32 Proximal mismatches [2MB] 33 Proximal mismatches [2MB] 34 Security: Anyone File name: Proximal mismatch (repeat 2).tif35 Content type: Data Description: PAGE image of restriction digest assay of promixal mismatches (used for figure 3) Mime-Type: image/tiff File size: 2MB

Proximal mismatches [2MB] 36 Proximal mismatches [2MB] 37 Proximal mismatches [2MB] 38 Security: Anyone File name: Proximal mismatch (repeat 3).tif39 Content type: Data Description: PAGE image of restriction digest assay of promixal mismatches (used for figure 3) Mime-Type: image/tiff File size: 2MB

12bp mismatches [2MB] 40 12bp mismatches [2MB] 41 12bp mismatches [2MB] 42 Security: Anyone File name: 12bp mismactch (repeat 1).tif43 Content type: Data Description: PAGE image of restriction digest assay of templates with 12bp mismatches at different positions (used for figure 4) Mime-Type: image/tiff File size: 2MB

12bp mismatches [2MB] 44 12bp mismatches [2MB] 45 12bp mismatches [2MB] 46 Security: Anyone File name: 12bp mismactch (repeat 2).tif47 Content type: Data Description: PAGE image of restriction digest assay of templates with 12bp mismatches at different positions (used for figure 4) Mime-Type: image/tiff File size: 2MB

12bp mismatches [2MB] 48 12bp mismatches [2MB] 49 12bp mismatches [2MB] 50 Security: Anyone File name: 12bp mismactch (repeat 3).tif51 Content type: Data Description: PAGE image of restriction digest assay of templates with 12bp mismatches at different positions (used for figure 4) Mime-Type: image/tiff File size: 2MB

Centered mismatches [2MB] 52 Centered mismatches [2MB] 53 Centered mismatches [2MB] 54 Security: Anyone File name: Centered mismatches.tif55 Content type: Data Description: PAGE image of restriction digest assay of centred mismatches (used for figure 6) Mime-Type: image/tiff File size: 2MB

Centered mismatches [2MB] 56 Centered mismatches [2MB] 57 Centered mismatches [2MB] 58 Security: Anyone File name: Centered mismatches (10 to 1).tif59 Content type: Data Description: PAGE image of restriction digest assay of centred mismatches using a ratio of filaments to template of 10:1 (used for figure S4) Mime-Type: image/tiff File size: 2MB

Centered mismatches [2MB] 60 Centered mismatches [2MB] 61 Centered mismatches [2MB] 62 Security: Anyone File name: Centered mismatches (20 to 1).tif63 Content type: Data Description: PAGE image of restriction digest assay of centred mismatches using a ratio of filaments to template of 20:1 (used for figure S4) Mime-Type: image/tiff File size: 2MB

Nicked templates [2MB] 64 Nicked templates [2MB] 65 Nicked templates [2MB] 66 Security: Anyone File name: Nicked templates (repeat 1).tif67 Content type: Data Description: PAGE image of restriction digest assay of templates with nicks at different locations (used for figure 6) Mime-Type: image/tiff File size: 2MB

Nicked templates [2MB] 68 Nicked templates [2MB] 69 Nicked templates [2MB] 70 Security: Anyone File name: Nicked templates (repeat 2).tif71 Content type: Data Description: PAGE image of restriction digest assay of templates with nicks at different locations (used for figure 6) Mime-Type: image/tiff File size: 2MB

Nicked templates [2MB] 72 Nicked templates [2MB] 73 Nicked templates [2MB] 74 Security: Anyone File name: Nicked templates (repeat 3).tif75 Content type: Data Description: PAGE image of restriction digest assay of templates with nicks at different locations (used for figure 6) Mime-Type: image/tiff File size: 2MB

Annealed templates [2MB] 76 Annealed templates [2MB] 77 Annealed templates [2MB] 78 Security: Anyone File name: Annealed templates.tif79 Content type: Data Description: PAGE image showing all used templates Mime-Type: image/tiff File size: 2MB

Control [2MB] 80 Control [2MB] 81 Control [2MB] 82 Security: Anyone File name: Complete digestion.tif83 Content type: Data Description: PAGE image showing complete digestion of template Mime-Type: image/tiff File size: 2MB

Control [2MB] 84 Control [2MB] 85 Control [2MB] 86 Security: Anyone File name: No digestion.tif87 Content type: Data Description: PAGE image showing no digestion of template Mime-Type: image/tiff File size: 2MB

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• 20. https://archive.researchdata.leeds.ac.uk/109/3/AFM%2020bp-mismatch%2025ng.001.tif
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• 27. https://archive.researchdata.leeds.ac.uk/109/4/AFM%2020bp-mismatch%2025ng.002.tif
• 28. https://archive.researchdata.leeds.ac.uk/109/5/Proximal%20mismatch%20%28repeat%201%29.tif
• 29. https://archive.researchdata.leeds.ac.uk/109/5/Proximal%20mismatch%20%28repeat%201%29.tif
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• 51. https://archive.researchdata.leeds.ac.uk/109/10/12bp%20mismactch%20%28repeat%203%29.tif
• 52. https://archive.researchdata.leeds.ac.uk/109/11/Centered%20mismatches.tif
• 53. https://archive.researchdata.leeds.ac.uk/109/11/Centered%20mismatches.tif
• 54. https://archive.researchdata.leeds.ac.uk/109/11/Centered%20mismatches.tif
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• 56. https://archive.researchdata.leeds.ac.uk/109/12/Centered%20mismatches%20%2810%20to%201%29.tif
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• 64. https://archive.researchdata.leeds.ac.uk/109/14/Nicked%20templates%20%28repeat%201%29.tif
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