1. ABOUT THE DATASET
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Title:	Data set for Probing RNA conformations using a polymer electrolyte solid-state nanopore

Creator(s): Chalmers Chau [1], Fabio Marcuccio [1], Dimitrios Soulias [1], Martin Andrew Edwards [2], Andrew Tuplin [1], Sheena E. Radford [1], Eric Hewitt [1], Paolo Actis [1]

Organisation(s): 1, University of Leeds; 2, University of Arkansas

Rights-holder(s): Chalmers Chau, Fabio Marcuccio, Dimitrios Soulias, Martin Andrew Edwards, Andrew Tuplin, Sheena E. Radford, Eric Hewitt, Paolo Actis

Publication Year: 2022

Description: The data set contains all ionic current traces recorded using pClamp system for the associated paper entitled: 'Probing RNA conformations using a polymer electrolyte solid-state nanopore'. The data is in the native pClamp format of abf files. Analysis routine can be performed with script that relies on abf files. The data set is aim to allow users to re-analysis the data and to reproduce the observations made in the assocaited publication.

Cite as: 
Chalmers Chau, Fabio Marcuccio, Dimitrios Soulias, Martin Andrew Edwards, Andrew Tuplin, Sheena E. Radford, Eric Hewitt, Paolo Actis (2022): Data set for Probing RNA conformations using a polymer electrolyte solid-state nanopore. [Dataset]. https://doi.org/10.5518/1251

Related publication: 
Chau C., Marcuccio F., Soulias D., Edwards M.A., Tuplin A., Radford S.E., Hewitt E., Actis P. Probing RNA conformations using a polymer electrolyte solid-state nanopore, ACS Nano, 2022 (Accepted pending reformatting).

Contact: p.actis@leeds.ac.uk; c.c.chau@leeds.ac.uk; e.w.hewitt@leeds.ac.uk


2. TERMS OF USE
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You may download to a local hard disk extracts for your personal and for non-commercial use only. 2022. Chalmers Chau, Fabio Marcuccio, Dimitrios Soulias, Martin Andrew Edwards, Andrew Tuplin, Sheena E. Radford, Eric Hewitt, Paolo Actis. This dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.]


3. FUNDING INFORMATION
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Funding organisation:
Engineering and Physical Science Research Council (EPSRC) UK,
Marie Skłodowska-Curie MSCA-ITN. single entity nanoelectrochemistry, SENTINEL project, 
Medical Research Council UK,
Royal Society Professorial Fellowship,
University of Leeds

Grant no.:
EP/W004933/1, 
812398, 
MR/N01054X/1,
RSRP\R1\211057


4. CONTENTS
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File listing

All files in this data set are in .abf format, this format can be accessed by pClamp software (any version), originlab software (any version), matlab or python with the pyABF package.

Below for the list of zip files:
Figure 1_effects of PEG molecular weight on analyte translocation behaviour
Figure 2_bath electrolyte dominates properties of translocation peaks
Figure 3_influences of the type of electrolytes
Figure 4_detection of RNA fragments with polymer electrolyte nanopore system
Figure 5_discrimination between co-transcriptionally and natively refolded RNA
Supporting Figure 4_translocation control
Supporting Figure 5_low-pass filter comparison
Supporting Figure 6_analyte translocation without low-pass filter
Supporting Figure 7_capture rate comparison
Supporting Figure 12_500 bp dsDNA traces


5. METHODS
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The ionic current trace was recorded using a MultiClamp 700B patch-clamp amplifier (Molecular Devices) in voltage-clamp mode via the associated software pClamp 10. For full method, please refer to the associated publication.