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Mosquito saliva enhances virus infection through sialokinin-dependent vascular leakage

Lefteri, Daniella A. and Bryden, Steven and Pingen, Marieke and Terry, Sandra and McCafferty, Ailish and Beswick, Emily F and Georgiev, Georgi and Van der Laan,, Marleen and Mastrullo, Valeria and Campagnolo, Paola and Waterhouse, Robert M and Varjak, Margus and Merits, Andres and Fragkoudis, Rennos and Griffin, Stephen and Shams, Kave and Pondeville, Emilie and McKimmie, Clive (2022) Mosquito saliva enhances virus infection through sialokinin-dependent vascular leakage. University of Leeds. [Dataset] https://doi.org/10.5518/1163

Dataset description

Viruses transmitted by Aedes mosquitoes are an increasingly important global cause of disease. Defining common determinants of host susceptibility to this large group of het- erogenous pathogens is key for informing the rational design of panviral medicines. Infection of the vertebrate host with these viruses is enhanced by mosquito saliva, a complex mixture of salivary-gland-derived factors and microbiota. We show that the enhancement of infection by saliva was dependent on vascular function and was inde- pendent of most antisaliva immune responses, including salivary microbiota. Instead, the Aedes gene product sialokinin mediated the enhancement of virus infection through a rapid reduction in endothelial barrier integrity. Sialokinin is unique within the insect world as having a vertebrate-like tachykinin sequence and is absent from nonvector competent Anopheles mosquitoes, whose saliva was not proviral and did not induce sim- ilar vascular permeability. Therapeutic strategies targeting sialokinin have the potential to limit disease severity following infection with Aedes-mosquito-borne viruses.

Keywords:	mosquitoes ; arbovirus ; inflammation ; endothelium
Subjects:	C000 - Biological sciences > C500 - Microbiology > C540 - Virology
Divisions:	Faculty of Medicine and Health > School of Medicine
Related resources:	Location Type https://eprints.whiterose.ac.uk/185862/ Publication https://doi.org/10.1073/pnas.2114309119 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	08 Jun 2022 13:32
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/974
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Lefteri, Daniella A. https://orcid.org/0000-0002-9985-4254 University of Leeds https://ror.org/024mrxd33 ror Bryden, Steven https://orcid.org/0000-0001-5408-6047 University of Leeds https://ror.org/024mrxd33 ror Pingen, Marieke https://orcid.org/0000-0001-5689-9076 University of Leeds https://ror.org/024mrxd33 ror Terry, Sandra University of Glasgow https://ror.org/00vtgdb53 ror McCafferty, Ailish https://orcid.org/0000-0002-4983-2014 University of Leeds https://ror.org/024mrxd33 ror Beswick, Emily F https://orcid.org/0000-0002-4129-5891 University of Leeds https://ror.org/024mrxd33 ror Georgiev, Georgi University of Leeds https://ror.org/024mrxd33 ror Van der Laan,, Marleen University of Leeds https://ror.org/024mrxd33 ror Mastrullo, Valeria https://orcid.org/0000-0002-6716-0370 University of Surrey https://ror.org/00ks66431 ror Campagnolo, Paola https://orcid.org/0000-0002-8957-3969 University of Surrey https://ror.org/00ks66431 ror Waterhouse, Robert M https://orcid.org/0000-0003-4199-9052 University of Lausanne https://ror.org/019whta54 ror Varjak, Margus https://orcid.org/0000-0003-2608-5148 University of Glasgow https://ror.org/00vtgdb53 ror Merits, Andres https://orcid.org/0000-0001-8193-0071 University of Tartu https://ror.org/03z77qz90 ror Fragkoudis, Rennos https://orcid.org/0000-0002-8451-2665 University of Edinburgh https://ror.org/01nrxwf90 ror Griffin, Stephen https://orcid.org/0000-0002-7233-5243 University of Leeds https://ror.org/024mrxd33 ror Shams, Kave https://orcid.org/0000-0002-8145-5351 University of Leeds https://ror.org/024mrxd33 ror Pondeville, Emilie https://orcid.org/0000-0001-8545-8472 University of Glasgow https://ror.org/00vtgdb53 ror McKimmie, Clive https://orcid.org/0000-0002-7694-9509 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: MRC, Wellcome Trust Collection period: Date from Date to 1 October 2017 30 September 2020 Grant numbers: MC_UU_12014/8, 108227/B/15/Z Publication date: 2022 Resource language: English Publisher: University of Leeds Contact Email Address: c.s.mckimmie@leeds.ac.uk Last Modified: 08 Jun 2022 22:35

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