Research Data Leeds Repository

Reverse genetics siRNA-based visual phenotype screen datasets identifying genes involved in supernumerary primary cilium formation

Basu, Basudha and Lake, Alice and Szymanska, Katarzyna and Wheway, Gabrielle and Bell, Sandra and Morrison, Ewan and Bond, Jacqueline and Johnson, Colin. A. (2021) Reverse genetics siRNA-based visual phenotype screen datasets identifying genes involved in supernumerary primary cilium formation. University of Leeds. [Dataset] https://doi.org/10.5518/1044

Dataset description

High content siRNA screen carried out to identify genes which when knocked-down, cause the formation of multiple primary cilia in the mIMCD3 cell-line. The dataset includes data from both the primary and secondary screens as separate files.

Keywords:	Cilia, high-content imaging
Subjects:	C000 - Biological sciences > C100 - Biology > C130 - Cell biology
Divisions:	Faculty of Medicine and Health > School of Medicine
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	18 Oct 2021 10:27
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/898
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Basu, Basudha https://orcid.org/0000-0002-5434-5202 University Of Leeds https://ror.org/024mrxd33 ROR Lake, Alice https://orcid.org/0000-0002-6341-8661 University Of Leeds https://ror.org/024mrxd33 ROR Massey University https://ror.org/052czxv31 ROR Szymanska, Katarzyna https://orcid.org/0000-0001-7736-5225 University Of Leeds https://ror.org/024mrxd33 ROR Wheway, Gabrielle https://orcid.org/0000-0002-0494-0783 University Hospital Southampton NHS Foundation Trust https://ror.org/0485axj58 ROR University of Southampton https://ror.org/01ryk1543 ROR Bell, Sandra https://orcid.org/0000-0002-3022-9864 University Of Leeds https://ror.org/024mrxd33 ROR Morrison, Ewan https://orcid.org/0000-0001-6269-6638 University Of Leeds https://ror.org/024mrxd33 ROR Bond, Jacqueline https://orcid.org/0000-0001-5390-5688 University Of Leeds https://ror.org/024mrxd33 ROR Johnson, Colin. A. https://orcid.org/0000-0002-2979-8234 University Of Leeds https://ror.org/024mrxd33 ROR Type of data: Dataset Research funders: MRC, BBSRC-SFI, European Community's Seventh Framework Programme FP7/2009 Grant numbers: MR/M000532/1, BB/P007791/1, 241955 SYSCILIA Publication date: 2021 Resource language: English Research project title: Functional genomics identification and characterization of novel disease egenes, mechanisms and pathways of ciliogenesis, Structure-function relationships in the ciliary transition zone, A systems biology approach to dissect cilia function and its disruption in human genetic disease Publisher: University of Leeds Contact Email Address: c.johnson@leeds.ac.uk Last Modified: 18 Oct 2021 22:35

Files

Data

Primary screen data [1MB] Primary screen data [1MB] Primary screen data [1MB] Visible to: Anyone File name: Primary_ciliascreen.txt Content type: Data Mime-Type: text/plain File size: 1MB

Secondary screen data [10kB] Secondary screen data [10kB] Secondary screen data [10kB] Visible to: Anyone File name: Secondary_ciliascreen.txt Content type: Data Mime-Type: text/plain File size: 10kB