Research Data Leeds Repository

Dataset for the study of “Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light‐Harvesting Proteins”

Meredith, Sophie A. and Yoneda, Takuro and Hancock, Ashley M. and Connell, Simon D. and Evans, Stephen D. and Morigaki, Kenichi and Adams, Peter G. (2021) Dataset for the study of “Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light‐Harvesting Proteins”. University of Leeds. [Dataset] https://doi.org/10.5518/884

Dataset description

This dataset shows the raw data, analysed data and documentation related to figures and tables from the study "Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light‐Harvesting Proteins". This includes: absorbance and fluorescence spectra; images from Fluorescence Lifetime Imaging Microscopy (FLIM); images from Atomic Force Microscopy measurements; epifluorescence measurements; analysis of fluorescence data (FLIM); other graphical analyses; tabulated numerical data.

Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C750 - Plant biochemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C760 - Biomolecular science C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science
Divisions:	Faculty of Biological Sciences > School of Biology Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.1002/smll.202006608 Publication http://eprints.whiterose.ac.uk/172993/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	23 Mar 2021 17:42
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/824
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Meredith, Sophie A. https://orcid.org/0000-0003-2550-9092 University Of Leeds https://ror.org/024mrxd33 ror Yoneda, Takuro Kobe University https://ror.org/03tgsfw79 ror Hancock, Ashley M. https://orcid.org/0000-0003-2069-5105 University Of Leeds https://ror.org/024mrxd33 ror Connell, Simon D. https://orcid.org/0000-0003-2500-5724 University Of Leeds https://ror.org/024mrxd33 ror Evans, Stephen D. https://orcid.org/0000-0001-8342-5335 University Of Leeds https://ror.org/024mrxd33 ror Morigaki, Kenichi https://orcid.org/0000-0002-2454-6513 Kobe University https://ror.org/03tgsfw79 ror Adams, Peter G. https://orcid.org/0000-0002-3940-8770 University Of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: The Royal Society, JSPS, BBSRC, EPSRC, EPSRC, MRC, EPSRC, JSPS, EPSRC Grant numbers: IEC/R3/18302, JPJSBP120195707, BB/M011151/1, EP/ J017566/1, EP/J017566/1, MR/M009084/1, EP/P023266/1, JPJSBP120195707, EP/T013958/1 Publication date: 2021 Resource language: English Metadata language: English Publisher: University of Leeds Contact Email Address: P.G.Adams@leeds.ac.uk Last Modified: 21 Apr 2021 08:06

Files

Documentation

ReadMe.txt [2kB] ReadMe.txt [2kB] ReadMe.txt [2kB] Visible to: Anyone File name: ReadMe.txt Content type: Documentation Mime-Type: text/plain File size: 2kB

Data

Diyne-PC templates FLIM [66MB] Diyne-PC templates FLIM [66MB] Diyne-PC templates FLIM [66MB] Visible to: Anyone File name: DiynePC_template.zip Content type: Data Description: FLIM data of polymerised diyne-PC templates Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 66MB

Diyne-PC templates AFM [61MB] Diyne-PC templates AFM [61MB] Diyne-PC templates AFM [61MB] Visible to: Anyone File name: DiynePC_template_AFM.zip Content type: Data Description: AFM data of polymerized diyne-PC templates. Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 61MB

Spectral overlap FLIM [8MB] Spectral overlap FLIM [8MB] Spectral overlap FLIM [8MB] Visible to: Anyone File name: Spectral_overlap.zip Content type: Data Description: FLIM data of polymerized diyne-PC templates, DOPC-TR bilayers and hybrid membranes organised into detection channels Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8MB

Spectral overlap calculations [21kB] Spectral overlap calculations [21kB] Spectral overlap calculations [21kB] Visible to: Anyone File name: Spectral_overlap_calculations.xlsx Content type: Data Description: Calculations for the spectral overlap between Diyne-PC/TR/Chl fluorescence channels of the FLIM Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 21kB

Excitation energy calculations [17kB] Excitation energy calculations [17kB] Excitation energy calculations [17kB] Visible to: Anyone File name: Excitation_energy_calculations.xlsx Content type: Data Description: Spreadsheet for calculating the excitation fluence for the FLIM system in Leeds Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 17kB

Hybrid membranes FLIM [768MB] Hybrid membranes FLIM [768MB] Hybrid membranes FLIM [768MB] Visible to: Anyone File name: Hybrid_membranes.zip Content type: Data Description: FLIM data of hybrid membranes across multiple preparations Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 768MB

Thylakoid extracts FLIM [24MB] Thylakoid extracts FLIM [24MB] Thylakoid extracts FLIM [24MB] Visible to: Anyone File name: Thy_extracts.zip Content type: Data Description: FLIM data of thylakoid extracts adhered to glass Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 24MB

Hybrid membranes AFM [115MB] Hybrid membranes AFM [115MB] Hybrid membranes AFM [115MB] Visible to: Anyone File name: Hybrid_membranes_AFM.zip Content type: Data Description: AFM data of hybrid membranes Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 115MB

Thylakoid extracts AFM [17MB] Thylakoid extracts AFM [17MB] Thylakoid extracts AFM [17MB] Visible to: Anyone File name: Thy_extracts_AFM.zip Content type: Data Description: AFM data of thylakoid extracts Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 17MB

Hybrid membranes correlated FLIM and AFM [75MB] Hybrid membranes correlated FLIM and AFM [75MB] Hybrid membranes correlated FLIM and AFM [75MB] Visible to: Anyone File name: Hybrid_membranes_FLIM_AFM.zip Content type: Data Description: Correlated FLIM and AFM data for hybrid membranes Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 75MB

Diyne PC templates correlated FLIM and AFM [52MB] Diyne PC templates correlated FLIM and AFM [52MB] Diyne PC templates correlated FLIM and AFM [52MB] Visible to: Anyone File name: DiynePC_template_FLIM_AFM.zip Content type: Data Description: Correlated FLIM and AFM data for empty diyne-PC templates Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 52MB

Hybrid membranes formation timelapse [48MB] Hybrid membranes formation timelapse [48MB] Hybrid membranes formation timelapse [48MB] Visible to: Anyone File name: Hybrid_membranes_formation_timelapse.zip Content type: Data Description: Timelapse FLIM data for hybrid membranes across multiple preparations Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 48MB

Hybrid membranes FRAP data [723MB] Hybrid membranes FRAP data [723MB] Hybrid membranes FRAP data [723MB] Visible to: Anyone File name: Hybrid_membranes_FRAP_data.zip Content type: Data Description: Epifluorescence FRAP experiments for hybrid membranes and DOPC-TR bilayers in patterned templates Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 723MB

FRAP mobile fraction analysis [17kB] FRAP mobile fraction analysis [17kB] FRAP mobile fraction analysis [17kB] Visible to: Anyone File name: FRAP_mobile_fraction_analysis.xlsx Content type: Data Description: Excel spreadsheet detailing the calculation of the mobile fraction in hybrid membranes and DOPC-TR bilayers in patterned templates Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 17kB

DOPC membranes AFM [32MB] DOPC membranes AFM [32MB] DOPC membranes AFM [32MB] Visible to: Anyone File name: DOPC_AFM.zip Content type: Data Description: AFM data of DOPC lipid membranes within diyne-PC templates Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 32MB

Photochemical assays FLIM [1GB] Photochemical assays FLIM [1GB] Photochemical assays FLIM [1GB] Visible to: Anyone File name: PC_assays.zip Content type: Data Description: FLIM images of hybrid membranes, TR membranes and LHCII proteoliposomes during stages of the photochemical assays Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Hybrid membranes full summary [27MB] Hybrid membranes full summary [27MB] Hybrid membranes full summary [27MB] Visible to: Anyone File name: Hybrid_membranes_full_summary.opju Content type: Data Description: Origin project containing all the image/file analysis and raw data from image profiles as displayed in graphical figures License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 27MB