Research Data Leeds Repository

De Novo Designed Peptide and Protein Hairpins Self-assemble into Sheets and Nanoparticles - dataset

Galloway, Johanna M. and Bray, Harriet E. V. and Shoemark, Deborah K. and Hodgson, Lorna R. and Coombs, Jennifer and Mantell, Judith M. and Rose, Ruth S. and Ross, James F. and Morris, Caroline and Harniman, Robert L. and Wood, Christopher W. and Arthur, Christopher and Verkade, Paul and Woolfson, Derek N. (2021) De Novo Designed Peptide and Protein Hairpins Self-assemble into Sheets and Nanoparticles - dataset. University of Leeds. [Dataset] https://doi.org/10.5518/955

Dataset description

AFM, TEM, fluorescence microscopy image files and spectral data published in DOI:10.1002/smll.202100472. Preprint of this is available at bioRxiv DOI:10.1101/2020.08.14.251462

Keywords:	coiled coil, computational modeling, peptide design, protein design, self-assembly
Divisions:	Faculty of Engineering and Physical Sciences > School of Chemistry
Related resources:	Location Type https://doi.org/10.1002/smll.202100472 Publication https://doi.org/10.1101/2020.08.14.251462 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	03 Feb 2021 09:53
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/811
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Galloway, Johanna M. https://orcid.org/0000-0003-3998-0870 University Of Leeds https://ror.org/024mrxd33 ROR University of Bristol Bray, Harriet E. V. University of Bristol https://ror.org/0524sp257 Shoemark, Deborah K. University of Bristol https://ror.org/0524sp257 Hodgson, Lorna R. University of Bristol https://ror.org/0524sp257 Coombs, Jennifer University of Bristol https://ror.org/0524sp257 Mantell, Judith M. University of Bristol https://ror.org/0524sp257 Rose, Ruth S. Queen Mary University of London https://ror.org/026zzn846 Ross, James F. University of Bristol https://ror.org/0524sp257 Morris, Caroline University of Bristol https://ror.org/0524sp257 Harniman, Robert L. University of Bristol https://ror.org/0524sp257 Wood, Christopher W. University of Bristol https://ror.org/0524sp257 Arthur, Christopher University of Bristol https://ror.org/0524sp257 Verkade, Paul https://orcid.org/0000-0002-2497-1026 University of Bristol https://ror.org/0524sp257 Woolfson, Derek N. University of Bristol https://ror.org/0524sp257 Type of data: Dataset Research funders: BBSRC, EPSRC Grant numbers: BB/M002969/1, BB/L014181/1, BB/L01386X/1, EP/K035746/1, EP/K03927X/1 Publication date: 2021 Resource language: English Publisher: University of Leeds Contact Email Address: j.m.galloway@leeds.ac.uk Last Modified: 03 Feb 2021 23:35

Files

Documentation

File listing for all zip files [6kB] File listing for all zip files [6kB] File listing for all zip files [6kB] Visible to: Anyone File name: zip_file_manifest.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6kB

Data

F2_TEM_peptide [135MB] F2_TEM_peptide [135MB] F2_TEM_peptide [135MB] Visible to: Anyone File name: F2_TEM_peptide.zip Content type: Data Description: TEM images and FFTs shown in Figure 2 separated files a to p Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 135MB

F3_AFM_peptide [316MB] F3_AFM_peptide [316MB] F3_AFM_peptide [316MB] Visible to: Anyone File name: F3_AFM_peptide.zip Content type: Data Description: AFM plots, processing and processed data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 316MB

F4_TEM_protein [52MB] F4_TEM_protein [52MB] F4_TEM_protein [52MB] Visible to: Anyone File name: F4_TEM_protein.zip Content type: Data Description: TEM images and FFTs shown in Figure 3 separated files a to j Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 52MB

S1_curvature_model [11kB] S1_curvature_model [11kB] S1_curvature_model [11kB] Visible to: Anyone File name: S1_curvature.xlsx Content type: Data Description: data plotted in fig S1 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 11kB

S2_nanospray_peptide [9MB] S2_nanospray_peptide [9MB] S2_nanospray_peptide [9MB] Visible to: Anyone File name: S2_peptide_nanospray_data.xlsx Content type: Data Description: spectra plotted in fig S2 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9MB

S3_HPLC_peptide [76kB] S3_HPLC_peptide [76kB] S3_HPLC_peptide [76kB] Visible to: Anyone File name: S3_HPLC_data.xlsx Content type: Data Description: absorbance plotted in fig S3 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 76kB

S4_AFM_sheet_1 [1MB] S4_AFM_sheet_1 [1MB] S4_AFM_sheet_1 [1MB] Visible to: Anyone File name: S4_AFM_sheet_1.zip Content type: Data Description: AFM plotted in fig S4 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

S5_AFM_sheet_2 [9MB] S5_AFM_sheet_2 [9MB] S5_AFM_sheet_2 [9MB] Visible to: Anyone File name: S5_AFM_sheet_2.zip Content type: Data Description: AFM plotted in fig S5 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9MB

S6_peptide_CLEM [110MB] S6_peptide_CLEM [110MB] S6_peptide_CLEM [110MB] Visible to: Anyone File name: S6_peptide_CLEM.zip Content type: Data Description: fluorescence and TEM plotted in fig S6 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 110MB

S8_PAGE_nanospray_protein [6MB] S8_PAGE_nanospray_protein [6MB] S8_PAGE_nanospray_protein [6MB] Visible to: Anyone File name: S8_PAGE_nanospray_protein.zip Content type: Data Description: spectra and SDS-PAGE plotted in figure S8 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6MB

S9_pro_HP-DT_dist_cells_CLEM [85MB] S9_pro_HP-DT_dist_cells_CLEM [85MB] S9_pro_HP-DT_dist_cells_CLEM [85MB] Visible to: Anyone File name: S9_pro_HP-DT_dist_cells_CLEM.zip Content type: Data Description: fluorescence and TEM plotted in fig S9 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 85MB

S10_pro_HP-DT_pH [235MB] S10_pro_HP-DT_pH [235MB] S10_pro_HP-DT_pH [235MB] Visible to: Anyone File name: S10_pro_HP-DT_pH.zip Content type: Data Description: TEM images and FFTs shown in fig S10 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 235MB

S11_proGFP_HP-DT_dist_cells_CLEM [103MB] S11_proGFP_HP-DT_dist_cells_CLEM [103MB] S11_proGFP_HP-DT_dist_cells_CLEM [103MB] Visible to: Anyone File name: S11_proGFP_HP-DT_dist_cells_CLEM.zip Content type: Data Description: fluorescence and TEM plotted in fig S11 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 103MB

S12_proGFP_HP-DT_dist_vitro_CLEM [66MB] S12_proGFP_HP-DT_dist_vitro_CLEM [66MB] S12_proGFP_HP-DT_dist_vitro_CLEM [66MB] Visible to: Anyone File name: S12_proGFP_HP-DT_dist_vitro_CLEM.zip Content type: Data Description: fluorescence and TEM plotted in fig S12 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 66MB