Research Data Leeds Repository

Single Molecule Protein Stabilisation Translates to Macromolecular Mechanics of Protein Network - Dataset

Hughes, Matt D.G. and Cussons, Sophie and Mahmoudi, Najet and Brockwell, David and Dougan, Lorna (2020) Single Molecule Protein Stabilisation Translates to Macromolecular Mechanics of Protein Network - Dataset. University of Leeds. [Dataset] https://doi.org/10.5518/799

Dataset description

Folded globular proteins are attractive building blocks for biopolymer-based materials, as their mechanically resistant structures carry out diverse biological functionality. While much is now understood about the mechanical response of single folded proteins, a major challenge is to understand and predictably control how single protein mechanics translates to the collective response of a network of connected folded proteins. Here, by utilising the binding of maltose to hydrogels constructed from photo-chemically cross-linked maltose binding protein (MBP), we investigate the effects of protein stabilisation at the molecular level on the macroscopic mechanical and structural properties of a protein-based hydrogel. Rheological measurements show an enhancement in the mechanical strength and energy dissipation of MBP hydrogels in the presence of maltose. Circular dichroism spectroscopy and differential scanning calorimetry measurements show that MBP remains both folded and functional in situ. By coupling these mechanical measurements with mesoscopic structural information obtained by small angle scattering, we propose an occupation model in which higher proportions of stabilised, ligand occupied, protein building blocks translate their increased stability to the macroscopic properties of the hydrogel network. This provides powerful opportunities to exploit environmentally responsive folded protein based biomaterials for many broad applications.

Keywords:	Hierarchical Mechanics, Protein Hydrogel, Biomaterial, Biophysics, Soft Matter
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.1039/C9SM02484K Publication http://eprints.whiterose.ac.uk/162393/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	24 Jun 2020 11:15
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/697
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Hughes, Matt D.G. https://orcid.org/0000-0001-5838-7939 University Of Leeds https://doi.org/10.13039/501100000777 crossref Cussons, Sophie https://orcid.org/0000-0002-9318-3859 University Of Leeds https://doi.org/10.13039/501100000777 crossref Mahmoudi, Najet https://orcid.org/0000-0003-4936-6911 STFC Rutherford Appleton Laboratory Brockwell, David https://orcid.org/0000-0002-0802-5937 University Of Leeds https://doi.org/10.13039/501100000777 crossref Dougan, Lorna https://orcid.org/0000-0002-2620-5827 University Of Leeds https://doi.org/10.13039/501100000777 crossref Type of data: Dataset Research funders: Biotechnology and Biological Sciences Research Council, Engineering and Physical Sciences Research Council Grant numbers: White Rose Industrial Biotechnology studentship network, EP/P020088X/1 Publication date: 2020 Resource language: English Metadata language: English Publisher: University of Leeds Contact Email Address: L.Dougan@leeds.ac.uk Last Modified: 07 Jul 2020 09:28

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Main Figure Data [690kB] Main Figure Data [690kB] Main Figure Data [690kB] Visible to: Anyone File name: main_figure_single_molecule_protein_stabilisation_translation.xlsx Content type: Data Description: Data Repository Main Figure Soft Matter - Single Molecule Protein Stabilisation Translation. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 690kB

Supplementary Figure Data [123kB] Supplementary Figure Data [123kB] Supplementary Figure Data [123kB] Visible to: Anyone File name: supp_figure_single_molecule_protein_stabilisation_translation.xlsx Content type: Data Description: Data Repository Supplementary Figure Soft Matter - Single Molecule Protein Stabilisation Translation Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 123kB